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- W2013288120 abstract "Pilosulin 1 is a synthetic 56–amino acid residue polypeptide that corresponds to the largest allergenic polypeptide found in the venom of the jumper ant Myrmecia pilosula. Initial experiments showed that pilosulin 1 lysed erythrocytes and killed proliferating B cells. Herein, we describe how flow cytometry was used to investigate the cytotoxicity of the peptide for human white blood cells. Cells were labeled with fluorochrome-conjugated antibodies, incubated with the peptide and 7-aminoactinomycin D (7-AAD), and then analyzed. The effects of varying the peptide concentration, serum concentration, incubation time, and incubation temperature were measured, and the cytotoxicity of pilosulin 1 was compared with that of the bee venom peptide melittin. The antibodies and the 7-AAD enabled the identification of cell subpopulations and dead cells, respectively. It was possible, using the appropriate mix of antibodies and four-color analysis, to monitor the killing of three or more cell subpopulations simultaneously. We found that 1) pilosulin 1 killed cells within minutes, with kinetics similar to those of melittin; 2) pilosulin 1 was a slightly more potent cytotoxic agent than melittin; 3) both pilosulin 1 and melittin were more potent against mononuclear leukocytes than against granulocytes; and 4) serum inhibited killing by either peptide. Cytometry 32:268–273, 1998. © 1998 Wiley-Liss, Inc." @default.
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- W2013288120 date "1998-08-01" @default.
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- W2013288120 title "Flow cytometric analysis of cell killing by the jumper ant venom peptide pilosulin 1" @default.
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- W2013288120 doi "https://doi.org/10.1002/(sici)1097-0320(19980801)32:4<268::aid-cyto2>3.0.co;2-e" @default.
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