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- W2013302383 abstract "Dispersion in connecting tubing represents a major contribution to extra-column band broadening in liquid chromatography, a factor that is particularly important in miniaturized high-performance liquid chromatographic systems. Although most analyses of extra-column effects are based on the Taylor—Aris theory of dispersion in tubes and the additivity of variances, these approaches are known to be inaccurate under some conditions, but alternative theoretical methods have not been verified experimentally. These aspects have been addressed by a combination of theory and experiment. Experimental elution curves in single tubes are shown to match closely the theoretical predictions based on solutions to the convective diffusion equation, without the need for any adjustable parameters. For tubes in series, use of a convolution relationship allows the accurate prediction of the response of the overall system when radial mixing between the two tubes is present. Although injection and detection systems have not been analysed in detail, they may contribute to discrepancies between theory and experiment if they are not well matched with the remainder of the chromatographic system." @default.
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- W2013302383 date "1991-09-01" @default.
- W2013302383 modified "2023-10-09" @default.
- W2013302383 title "Dispersion in round tubes and its implications for extracolumn dispersion" @default.
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- W2013302383 doi "https://doi.org/10.1016/s0021-9673(01)96224-7" @default.
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