FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013313482> ?p ?o ?g. }

• W2013313482 endingPage "5511" @default.
• W2013313482 startingPage "5504" @default.
• W2013313482 abstract "An array of eight porous monolithic columns, prepared in a Zeonor polymeric chip by UV-initiated polymerization of butyl methacrylate and ethylene dimethacrylate, was tested for solid-phase extraction (SPE) cleanup of biological samples prior to directly coupled electrospray mass spectrometry (ESI-MS). The chip, fabricated by hot embossing and thermal bonding, consists of eight parallel channels (10 mm long, 360 microm i.d.) connected via external fused-silica capillaries. The monomer mixture was aspirated simultaneously into the eight channels using a homemade vacuum manifold device and polymerized in parallel for 20 min under UV irradiation. The porous monolithic columns were then characterized by scanning electron microscopy and evaluated by ESI-MS applications with respect to sample capacity, recovery, reproducibility of peak area or peak height ratios, and linearity between peak height ratio and concentration using imipramine as a pharmaceutical test compound. The average sample capacity was estimated to be 0.30 microg with a relative standard deviation (RSD) of 26.5% for the eight monolithic columns on the same polymeric chip. For two chips prepared using the same monomer mixture, the difference in average sample capacity was 7.0%. The average recovery for the eight monolithic SPE columns on the same chip was 79.1% with an RSD of 7.9%. Using imipramine-d3 as an internal standard, the RSD of peak height ratios for the eight different columns was 2.0% for a standard solution containing 1 microg/mL imipramine. A linear calibration curve (R2 = 0.9995) was obtained for standard aqueous solutions of imipramine in the range from 0.025 to 10 microg/mL. To demonstrate the analytical potential of the chip-based SPE system, two different types of real-world samples including human urine sample and P450 drug metabolism incubation mixture were tested. Similar to standard aqueous solution, a linear correlation (R2 = 0.9995) was also found for human urine sample spiked with imipramine in the range of 0.025-10 microg/ mL. When aliquots of a human urine sample spiked with 1 microg/mL imipramine were loaded onto eight different monolithic columns, the RSD of peak height ratios was 3.8%. For a P450-imipramine incubation mixture, the formation of the N-demethylated metabolite (m/z 267.2) and the monohydroxylated metabolite (m/z 297.2) of imipramine was observed following chip-based monolithic SPE sample cleanup and preconcentration." @default.
• W2013313482 created "2016-06-24" @default.
• W2013313482 creator A5031709603 @default.
• W2013313482 creator A5046182624 @default.
• W2013313482 creator A5054043854 @default.
• W2013313482 date "2003-09-12" @default.
• W2013313482 modified "2023-10-10" @default.
• W2013313482 title "Chip-Based Solid-Phase Extraction Pretreatment for Direct Electrospray Mass Spectrometry Analysis Using an Array of Monolithic Columns in a Polymeric Substrate" @default.
• W2013313482 cites W1971194063 @default.
• W2013313482 cites W1987358962 @default.
• W2013313482 cites W1994348463 @default.
• W2013313482 cites W2002887234 @default.
• W2013313482 cites W2003614295 @default.
• W2013313482 cites W2015630401 @default.
• W2013313482 cites W2021337135 @default.
• W2013313482 cites W2024174655 @default.
• W2013313482 cites W2034371883 @default.
• W2013313482 cites W2038257421 @default.
• W2013313482 cites W2042114357 @default.
• W2013313482 cites W2043458312 @default.
• W2013313482 cites W2051007271 @default.
• W2013313482 cites W2058449444 @default.
• W2013313482 cites W2062838726 @default.
• W2013313482 cites W2079714627 @default.
• W2013313482 cites W2094874581 @default.
• W2013313482 cites W2122403774 @default.
• W2013313482 cites W2139751673 @default.
• W2013313482 cites W2150704355 @default.
• W2013313482 cites W2154883443 @default.
• W2013313482 cites W2170543233 @default.
• W2013313482 doi "https://doi.org/10.1021/ac030196w" @default.
• W2013313482 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14710831" @default.
• W2013313482 hasPublicationYear "2003" @default.
• W2013313482 type Work @default.
• W2013313482 sameAs 2013313482 @default.
• W2013313482 citedByCount "97" @default.
• W2013313482 countsByYear W20133134822012 @default.
• W2013313482 countsByYear W20133134822013 @default.
• W2013313482 countsByYear W20133134822014 @default.
• W2013313482 countsByYear W20133134822015 @default.
• W2013313482 countsByYear W20133134822016 @default.
• W2013313482 countsByYear W20133134822017 @default.
• W2013313482 countsByYear W20133134822018 @default.
• W2013313482 countsByYear W20133134822020 @default.
• W2013313482 countsByYear W20133134822021 @default.
• W2013313482 crossrefType "journal-article" @default.
• W2013313482 hasAuthorship W2013313482A5031709603 @default.
• W2013313482 hasAuthorship W2013313482A5046182624 @default.
• W2013313482 hasAuthorship W2013313482A5054043854 @default.
• W2013313482 hasConcept C113196181 @default.
• W2013313482 hasConcept C134871310 @default.
• W2013313482 hasConcept C159985019 @default.
• W2013313482 hasConcept C162356407 @default.
• W2013313482 hasConcept C178790620 @default.
• W2013313482 hasConcept C179998833 @default.
• W2013313482 hasConcept C185592680 @default.
• W2013313482 hasConcept C192562407 @default.
• W2013313482 hasConcept C194451485 @default.
• W2013313482 hasConcept C26771246 @default.
• W2013313482 hasConcept C43617362 @default.
• W2013313482 hasConcept C44228677 @default.
• W2013313482 hasConcept C4725764 @default.
• W2013313482 hasConcept C521977710 @default.
• W2013313482 hasConcept C81426234 @default.
• W2013313482 hasConceptScore W2013313482C113196181 @default.
• W2013313482 hasConceptScore W2013313482C134871310 @default.
• W2013313482 hasConceptScore W2013313482C159985019 @default.
• W2013313482 hasConceptScore W2013313482C162356407 @default.
• W2013313482 hasConceptScore W2013313482C178790620 @default.
• W2013313482 hasConceptScore W2013313482C179998833 @default.
• W2013313482 hasConceptScore W2013313482C185592680 @default.
• W2013313482 hasConceptScore W2013313482C192562407 @default.
• W2013313482 hasConceptScore W2013313482C194451485 @default.
• W2013313482 hasConceptScore W2013313482C26771246 @default.
• W2013313482 hasConceptScore W2013313482C43617362 @default.
• W2013313482 hasConceptScore W2013313482C44228677 @default.
• W2013313482 hasConceptScore W2013313482C4725764 @default.
• W2013313482 hasConceptScore W2013313482C521977710 @default.
• W2013313482 hasConceptScore W2013313482C81426234 @default.
• W2013313482 hasIssue "20" @default.
• W2013313482 hasLocation W20133134821 @default.
• W2013313482 hasLocation W20133134822 @default.
• W2013313482 hasOpenAccess W2013313482 @default.
• W2013313482 hasPrimaryLocation W20133134821 @default.
• W2013313482 hasRelatedWork W1984111050 @default.
• W2013313482 hasRelatedWork W2017717062 @default.
• W2013313482 hasRelatedWork W2047477686 @default.
• W2013313482 hasRelatedWork W2077738015 @default.
• W2013313482 hasRelatedWork W2093681008 @default.
• W2013313482 hasRelatedWork W2545638916 @default.
• W2013313482 hasRelatedWork W2606359044 @default.
• W2013313482 hasRelatedWork W3024835196 @default.
• W2013313482 hasRelatedWork W3207818033 @default.
• W2013313482 hasRelatedWork W4296016702 @default.
• W2013313482 hasVolume "75" @default.
• W2013313482 isParatext "false" @default.
• W2013313482 isRetracted "false" @default.
• W2013313482 magId "2013313482" @default.

• next