FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013316254> ?p ?o ?g. }

• W2013316254 endingPage "4984" @default.
• W2013316254 startingPage "4976" @default.
• W2013316254 abstract "The potential of integration of functions in microfluidic chips is demonstrated by implementing on-chip preconcentration of proteins prior to on-chip protein sizing by sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS−PAGE). Two polymeric elementsa thin (∼50 μm) size exclusion membrane for preconcentration and a longer (∼cm) porous monolith for protein sizingwere fabricated in situ using photopolymerization. Contiguous placement of the two polymeric elements in the channels of a microchip enabled simple and zero dead volume integration of the preconcentration with SDS−PAGE. The size exclusion membrane was polymerized in the injection channel using a shaped laser beam, and the sizing monolith was cast by photolithography using a mask and UV lamp. Proteins injected electrophoretically were trapped on the upstream side of the size exclusion membrane (MW cutoff ∼10 kDa) and eluted off the membrane by reversing the electric field. Subsequently, the concentrated proteins were separated in a cross-linked polyacrylamide monolith that was patterned contiguous to the size exclusion membrane. The extent of protein preconcentration is easily tuned by varying the voltage during injection or by controlling the sample volume loaded. Electric fields applied across the nanoporous membrane resulted in a concentration polarization effect evidenced by decreasing current over time and irreproducible migration of proteins during sizing. To minimize the concentration polarization effect, sieving gels were polymerized only on the separation side of the membrane, and an alternate electrical current path was employed, bypassing the membrane, for most of the elution and separation steps. Electrophoretically sweeping a fixed sample volume against the membrane yields preconcentration factors that are independent of protein mobility. The volume sweeping method also avoids biased protein loading from concentration polarization and sample matrix variations. Mobilities of the concentrated proteins were log−linear with respect to molecular weight, demonstrating the suitability of this approach for protein sizing. Proteins were concentrated rapidly (<5 min) over 1000-fold followed by high-resolution separation in the sieving monolith. Proteins with concentrations as low as 50 fM were detectable with 30 min of preconcentration time. The integrated preconcentration−sizing approach facilitates analysis of low-abundant proteins that cannot be otherwise detected. Moreover, the integrated preconcentration−analysis approach employing in situ formation of photopatterned polymeric elements provides a generic, inexpensive, and versatile method to integrate functions at chip level and can be extended to lowering of detection limits for other applications such as DNA analysis and clinical diagnostics." @default.
• W2013316254 created "2016-06-24" @default.
• W2013316254 creator A5041375274 @default.
• W2013316254 creator A5046265360 @default.
• W2013316254 creator A5049492000 @default.
• W2013316254 creator A5064883605 @default.
• W2013316254 creator A5068709566 @default.
• W2013316254 date "2006-05-23" @default.
• W2013316254 modified "2023-10-02" @default.
• W2013316254 title "Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels" @default.
• W2013316254 cites W1966737442 @default.
• W2013316254 cites W1971194063 @default.
• W2013316254 cites W1988446840 @default.
• W2013316254 cites W1989914985 @default.
• W2013316254 cites W1990971449 @default.
• W2013316254 cites W1997313982 @default.
• W2013316254 cites W2003614295 @default.
• W2013316254 cites W2009283704 @default.
• W2013316254 cites W2010280805 @default.
• W2013316254 cites W2013117405 @default.
• W2013316254 cites W2023994776 @default.
• W2013316254 cites W2028089203 @default.
• W2013316254 cites W2029254802 @default.
• W2013316254 cites W2029784517 @default.
• W2013316254 cites W2036966781 @default.
• W2013316254 cites W2038706415 @default.
• W2013316254 cites W2039533283 @default.
• W2013316254 cites W2050584354 @default.
• W2013316254 cites W2064808990 @default.
• W2013316254 cites W2065293530 @default.
• W2013316254 cites W2066818093 @default.
• W2013316254 cites W2070561610 @default.
• W2013316254 cites W2076737532 @default.
• W2013316254 cites W2083695757 @default.
• W2013316254 cites W2089058681 @default.
• W2013316254 cites W2091718555 @default.
• W2013316254 cites W2121005086 @default.
• W2013316254 cites W2144587906 @default.
• W2013316254 cites W2318138025 @default.
• W2013316254 cites W327027320 @default.
• W2013316254 doi "https://doi.org/10.1021/ac0600454" @default.
• W2013316254 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16841920" @default.
• W2013316254 hasPublicationYear "2006" @default.
• W2013316254 type Work @default.
• W2013316254 sameAs 2013316254 @default.
• W2013316254 citedByCount "161" @default.
• W2013316254 countsByYear W20133162542012 @default.
• W2013316254 countsByYear W20133162542013 @default.
• W2013316254 countsByYear W20133162542014 @default.
• W2013316254 countsByYear W20133162542015 @default.
• W2013316254 countsByYear W20133162542016 @default.
• W2013316254 countsByYear W20133162542017 @default.
• W2013316254 countsByYear W20133162542018 @default.
• W2013316254 countsByYear W20133162542019 @default.
• W2013316254 countsByYear W20133162542020 @default.
• W2013316254 countsByYear W20133162542021 @default.
• W2013316254 countsByYear W20133162542022 @default.
• W2013316254 countsByYear W20133162542023 @default.
• W2013316254 crossrefType "journal-article" @default.
• W2013316254 hasAuthorship W2013316254A5041375274 @default.
• W2013316254 hasAuthorship W2013316254A5046265360 @default.
• W2013316254 hasAuthorship W2013316254A5049492000 @default.
• W2013316254 hasAuthorship W2013316254A5064883605 @default.
• W2013316254 hasAuthorship W2013316254A5068709566 @default.
• W2013316254 hasConcept C113196181 @default.
• W2013316254 hasConcept C152815550 @default.
• W2013316254 hasConcept C161790260 @default.
• W2013316254 hasConcept C171250308 @default.
• W2013316254 hasConcept C178790620 @default.
• W2013316254 hasConcept C185592680 @default.
• W2013316254 hasConcept C192562407 @default.
• W2013316254 hasConcept C2776317363 @default.
• W2013316254 hasConcept C2778913249 @default.
• W2013316254 hasConcept C40250595 @default.
• W2013316254 hasConcept C41625074 @default.
• W2013316254 hasConcept C43617362 @default.
• W2013316254 hasConcept C44228677 @default.
• W2013316254 hasConcept C521977710 @default.
• W2013316254 hasConcept C55493867 @default.
• W2013316254 hasConcept C73826308 @default.
• W2013316254 hasConcept C8673954 @default.
• W2013316254 hasConceptScore W2013316254C113196181 @default.
• W2013316254 hasConceptScore W2013316254C152815550 @default.
• W2013316254 hasConceptScore W2013316254C161790260 @default.
• W2013316254 hasConceptScore W2013316254C171250308 @default.
• W2013316254 hasConceptScore W2013316254C178790620 @default.
• W2013316254 hasConceptScore W2013316254C185592680 @default.
• W2013316254 hasConceptScore W2013316254C192562407 @default.
• W2013316254 hasConceptScore W2013316254C2776317363 @default.
• W2013316254 hasConceptScore W2013316254C2778913249 @default.
• W2013316254 hasConceptScore W2013316254C40250595 @default.
• W2013316254 hasConceptScore W2013316254C41625074 @default.
• W2013316254 hasConceptScore W2013316254C43617362 @default.
• W2013316254 hasConceptScore W2013316254C44228677 @default.
• W2013316254 hasConceptScore W2013316254C521977710 @default.
• W2013316254 hasConceptScore W2013316254C55493867 @default.
• W2013316254 hasConceptScore W2013316254C73826308 @default.
• W2013316254 hasConceptScore W2013316254C8673954 @default.

• next