FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013318735> ?p ?o ?g. }

• W2013318735 endingPage "17" @default.
• W2013318735 startingPage "15" @default.
• W2013318735 abstract "Aims: To identify peptides that mimic (mimotopes) conformational and protective epitopes of RSV fusion protein and to assess their efficacy as immunogens and potential vaccines. Material and methods: An 8-mer solid-phase (TG resin) library was screened with a neutralising and protective RSV fusion protein specific monoclonal antibodies (Mab-19). After selection of positive beads, reactive sequences were identified by microsequencing and 8-mer peptides were synthesised. Improvement of binding was analysed by amino acid replacement using the SPOTs method. Results: Mabs were not able to bind to the free and soluble peptides, nor did these peptides induce anti-RSV specific antibodies. However, several peptides re-synthesised on a TG resin (to produce de-protected 8-mer peptides linked to the resin) or as SPOTs reacted specifically. Therefore it was critical to be able to reproduce this conformation in order to use these mimotopes as immunogens and potential vaccines. Using C-terminal constrained versions of the mimotopes, strong binding of one of the Mabs to the peptides was demonstrated by surface-plasmon resonance. Immunisation of Balb/c mice with these peptide-mimics produced anti-sera that: (1) reacted specifically with RSV; (2) inhibited the binding of the Mab to the virus; (3) neutralised RSV in vitro with high titres (range: 80–640); and (4) reduce significantly the viral load in the lungs of mice challenged with RSV (P<0.01). Conclusions: This report demonstrates for the first time that: (1) a protective epitope of the conserved RSV fusion protein can be mimicked by synthetic peptides; and (2) immunisations with these mimotopes induced specific anti-RSV neutralising antibodies and reduced viral load in vivo. These results represent a novel concept for the development of a vaccine against RSV." @default.
• W2013318735 created "2016-06-24" @default.
• W2013318735 creator A5010983441 @default.
• W2013318735 creator A5041765515 @default.
• W2013318735 creator A5044053285 @default.
• W2013318735 creator A5044716018 @default.
• W2013318735 creator A5048538844 @default.
• W2013318735 creator A5053470697 @default.
• W2013318735 creator A5056929517 @default.
• W2013318735 date "1997-06-01" @default.
• W2013318735 modified "2023-09-25" @default.
• W2013318735 title "Peptide mimics of a conformationally constrained protective epitopes of respiratory syncytial virus fusion protein" @default.
• W2013318735 cites W1887285520 @default.
• W2013318735 cites W1986797312 @default.
• W2013318735 cites W2002985673 @default.
• W2013318735 cites W2006319694 @default.
• W2013318735 cites W2006446936 @default.
• W2013318735 cites W2010649076 @default.
• W2013318735 cites W2012958292 @default.
• W2013318735 cites W2029950889 @default.
• W2013318735 cites W2043619738 @default.
• W2013318735 cites W2047084985 @default.
• W2013318735 cites W2057761745 @default.
• W2013318735 cites W2063655237 @default.
• W2013318735 cites W2072303166 @default.
• W2013318735 cites W2082887166 @default.
• W2013318735 cites W2089421123 @default.
• W2013318735 cites W2105574393 @default.
• W2013318735 cites W2121497936 @default.
• W2013318735 cites W2133582708 @default.
• W2013318735 cites W2133724729 @default.
• W2013318735 cites W2142746683 @default.
• W2013318735 cites W2152269583 @default.
• W2013318735 cites W2156501592 @default.
• W2013318735 cites W2164869674 @default.
• W2013318735 cites W2164992622 @default.
• W2013318735 cites W2167277992 @default.
• W2013318735 cites W2253477296 @default.
• W2013318735 cites W2328116798 @default.
• W2013318735 cites W2417598625 @default.
• W2013318735 cites W1652302321 @default.
• W2013318735 doi "https://doi.org/10.1016/s0165-2478(97)00045-x" @default.
• W2013318735 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9232419" @default.
• W2013318735 hasPublicationYear "1997" @default.
• W2013318735 type Work @default.
• W2013318735 sameAs 2013318735 @default.
• W2013318735 citedByCount "8" @default.
• W2013318735 crossrefType "journal-article" @default.
• W2013318735 hasAuthorship W2013318735A5010983441 @default.
• W2013318735 hasAuthorship W2013318735A5041765515 @default.
• W2013318735 hasAuthorship W2013318735A5044053285 @default.
• W2013318735 hasAuthorship W2013318735A5044716018 @default.
• W2013318735 hasAuthorship W2013318735A5048538844 @default.
• W2013318735 hasAuthorship W2013318735A5053470697 @default.
• W2013318735 hasAuthorship W2013318735A5056929517 @default.
• W2013318735 hasConcept C104317684 @default.
• W2013318735 hasConcept C123894998 @default.
• W2013318735 hasConcept C153911025 @default.
• W2013318735 hasConcept C159047783 @default.
• W2013318735 hasConcept C159654299 @default.
• W2013318735 hasConcept C167625842 @default.
• W2013318735 hasConcept C185592680 @default.
• W2013318735 hasConcept C186268636 @default.
• W2013318735 hasConcept C195616568 @default.
• W2013318735 hasConcept C202751555 @default.
• W2013318735 hasConcept C203014093 @default.
• W2013318735 hasConcept C2522874641 @default.
• W2013318735 hasConcept C2776471321 @default.
• W2013318735 hasConcept C2779281246 @default.
• W2013318735 hasConcept C2781241564 @default.
• W2013318735 hasConcept C40767141 @default.
• W2013318735 hasConcept C542903549 @default.
• W2013318735 hasConcept C55493867 @default.
• W2013318735 hasConcept C86803240 @default.
• W2013318735 hasConceptScore W2013318735C104317684 @default.
• W2013318735 hasConceptScore W2013318735C123894998 @default.
• W2013318735 hasConceptScore W2013318735C153911025 @default.
• W2013318735 hasConceptScore W2013318735C159047783 @default.
• W2013318735 hasConceptScore W2013318735C159654299 @default.
• W2013318735 hasConceptScore W2013318735C167625842 @default.
• W2013318735 hasConceptScore W2013318735C185592680 @default.
• W2013318735 hasConceptScore W2013318735C186268636 @default.
• W2013318735 hasConceptScore W2013318735C195616568 @default.
• W2013318735 hasConceptScore W2013318735C202751555 @default.
• W2013318735 hasConceptScore W2013318735C203014093 @default.
• W2013318735 hasConceptScore W2013318735C2522874641 @default.
• W2013318735 hasConceptScore W2013318735C2776471321 @default.
• W2013318735 hasConceptScore W2013318735C2779281246 @default.
• W2013318735 hasConceptScore W2013318735C2781241564 @default.
• W2013318735 hasConceptScore W2013318735C40767141 @default.
• W2013318735 hasConceptScore W2013318735C542903549 @default.
• W2013318735 hasConceptScore W2013318735C55493867 @default.
• W2013318735 hasConceptScore W2013318735C86803240 @default.
• W2013318735 hasIssue "1-3" @default.
• W2013318735 hasLocation W20133187351 @default.
• W2013318735 hasLocation W20133187352 @default.
• W2013318735 hasOpenAccess W2013318735 @default.
• W2013318735 hasPrimaryLocation W20133187351 @default.

• next