Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013341646> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2013341646 endingPage "51" @default.
- W2013341646 startingPage "47" @default.
- W2013341646 abstract "Signal transduction assays, particularly for protein kinases, are an area of increasing interest and activity for laboratories investigating the regulation of cellular functions. The traditional kinase assay methods require the tedious and time consuming manipulation of phosphocellulose disks typically used to bind the phosphorylated substrate. Drug discovery research requires the availability of rapid and reliable procedures to evaluate large numbers of samples for bioactivity. The 96-well phosphocellulose MultiScreen(r) assay plates were specifically developed to meet these assay requirements. A cyclic-AMP-dependent protein kinase A (PKA) assay was optimized to be performed entirely within the phosphocellulose MultiScreen plate, including reagent and sample addition, incubation, washing, and direct microplate scintillation counting. The protocol is directly adaptable to a high throughput kinase screen. Both the Kemptide peptide (Leu-Arg-Arg-Ala-Ser-Leu-Gly) and the histone H1 protein were used as the phosphorylation substrates. Crude and purified PKA enzymes were found to have a sensitivity of 0.4 U for Kemptide substrate, which was comparable to the assay performed by the traditional transfer to phosphocellulose paper. The results demonstrate that kinase assays can be performed entirely in a MultiScreen phosphocellulose plate." @default.
- W2013341646 created "2016-06-24" @default.
- W2013341646 creator A5028784059 @default.
- W2013341646 creator A5084813252 @default.
- W2013341646 date "1996-02-01" @default.
- W2013341646 modified "2023-10-14" @default.
- W2013341646 title "High Throughput Screening Protein Kinase Assays Optimized for Reaction, Binding, and Detection Totally within a 96-Well Plate" @default.
- W2013341646 cites W1572485476 @default.
- W2013341646 cites W1596261235 @default.
- W2013341646 cites W1973179766 @default.
- W2013341646 cites W1975068500 @default.
- W2013341646 cites W1976667813 @default.
- W2013341646 cites W2002677991 @default.
- W2013341646 cites W2014465746 @default.
- W2013341646 cites W2035521935 @default.
- W2013341646 cites W2064520079 @default.
- W2013341646 cites W4211062907 @default.
- W2013341646 doi "https://doi.org/10.1177/108705719600100115" @default.
- W2013341646 hasPublicationYear "1996" @default.
- W2013341646 type Work @default.
- W2013341646 sameAs 2013341646 @default.
- W2013341646 citedByCount "13" @default.
- W2013341646 countsByYear W20133416462012 @default.
- W2013341646 countsByYear W20133416462019 @default.
- W2013341646 countsByYear W20133416462021 @default.
- W2013341646 crossrefType "journal-article" @default.
- W2013341646 hasAuthorship W2013341646A5028784059 @default.
- W2013341646 hasAuthorship W2013341646A5084813252 @default.
- W2013341646 hasBestOaLocation W20133416461 @default.
- W2013341646 hasConcept C153911025 @default.
- W2013341646 hasConcept C157764524 @default.
- W2013341646 hasConcept C184235292 @default.
- W2013341646 hasConcept C185592680 @default.
- W2013341646 hasConcept C41008148 @default.
- W2013341646 hasConcept C43617362 @default.
- W2013341646 hasConcept C51323132 @default.
- W2013341646 hasConcept C55493867 @default.
- W2013341646 hasConcept C555944384 @default.
- W2013341646 hasConcept C70721500 @default.
- W2013341646 hasConcept C76155785 @default.
- W2013341646 hasConcept C86803240 @default.
- W2013341646 hasConceptScore W2013341646C153911025 @default.
- W2013341646 hasConceptScore W2013341646C157764524 @default.
- W2013341646 hasConceptScore W2013341646C184235292 @default.
- W2013341646 hasConceptScore W2013341646C185592680 @default.
- W2013341646 hasConceptScore W2013341646C41008148 @default.
- W2013341646 hasConceptScore W2013341646C43617362 @default.
- W2013341646 hasConceptScore W2013341646C51323132 @default.
- W2013341646 hasConceptScore W2013341646C55493867 @default.
- W2013341646 hasConceptScore W2013341646C555944384 @default.
- W2013341646 hasConceptScore W2013341646C70721500 @default.
- W2013341646 hasConceptScore W2013341646C76155785 @default.
- W2013341646 hasConceptScore W2013341646C86803240 @default.
- W2013341646 hasIssue "1" @default.
- W2013341646 hasLocation W20133416461 @default.
- W2013341646 hasOpenAccess W2013341646 @default.
- W2013341646 hasPrimaryLocation W20133416461 @default.
- W2013341646 hasRelatedWork W2047697325 @default.
- W2013341646 hasRelatedWork W2065115901 @default.
- W2013341646 hasRelatedWork W2097654801 @default.
- W2013341646 hasRelatedWork W2347855695 @default.
- W2013341646 hasRelatedWork W2356775162 @default.
- W2013341646 hasRelatedWork W2593805794 @default.
- W2013341646 hasRelatedWork W3150498631 @default.
- W2013341646 hasRelatedWork W4231807434 @default.
- W2013341646 hasRelatedWork W580487332 @default.
- W2013341646 hasRelatedWork W2513162865 @default.
- W2013341646 hasVolume "1" @default.
- W2013341646 isParatext "false" @default.
- W2013341646 isRetracted "false" @default.
- W2013341646 magId "2013341646" @default.
- W2013341646 workType "article" @default.