Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013357667> ?p ?o ?g. }
- W2013357667 endingPage "880" @default.
- W2013357667 startingPage "874" @default.
- W2013357667 abstract "A mode of internal motion of single tryptophan, Trp 86, of Streptomyces subtilisin inhibitor, was analyzed from its time-resolved fluorescence. The intensity and anisotropy decays of Trp 86 were measured in the picosecond range. These decays were analyzed with theoretical expressions derived assuming that the indole ring of tryptophan as an asymmetric rotor rotates around covalent bonds connecting indole with the peptide chain and an effective quencher of fluorescence of Trp 86 is the nearby SS bond of Cys 35-Cys 50. First, the intensity decays at 6 degrees, 20 degrees, and 40 degrees C were analyzed, and then the both decays of the intensity and anisotropy at 20 degrees C were simultaneously simulated with common parameters. Constants concerning geometrical structures of the protein used for the analysis were obtained from x-ray crystallographic data. Best fit between the observed and calculated decay curves was obtained by a nonlinear least squares method by adjusting a quenching constant averaged over the rotational angles, koq height of the potential energy, p, and three of six diffusion coefficients, Dxx, Dyy, Dzz, Dxy, Dyz, and Dzx, as variable parameters. The obtained results revealed that the internal motion of the indole ring became faster, the quenching rate of the fluorescence of Trp 86 was enhanced and the height of potential energy became lower at higher temperatures, and suggested that Trp 86 was wobbling around the long axis of the indole ring in the protein." @default.
- W2013357667 created "2016-06-24" @default.
- W2013357667 creator A5002137973 @default.
- W2013357667 creator A5028243612 @default.
- W2013357667 creator A5031049857 @default.
- W2013357667 creator A5034691817 @default.
- W2013357667 creator A5066073351 @default.
- W2013357667 date "1994-08-01" @default.
- W2013357667 modified "2023-10-18" @default.
- W2013357667 title "Analysis of internal motion of single tryptophan in Streptomyces subtilisin inhibitor from its picosecond time-resolved fluorescence" @default.
- W2013357667 cites W1837261016 @default.
- W2013357667 cites W1974292841 @default.
- W2013357667 cites W1976600619 @default.
- W2013357667 cites W1978793878 @default.
- W2013357667 cites W1982414965 @default.
- W2013357667 cites W2007833676 @default.
- W2013357667 cites W2016112786 @default.
- W2013357667 cites W2017267173 @default.
- W2013357667 cites W2040350133 @default.
- W2013357667 cites W2067879554 @default.
- W2013357667 cites W2074904307 @default.
- W2013357667 cites W2075483304 @default.
- W2013357667 cites W2093059489 @default.
- W2013357667 cites W2120997075 @default.
- W2013357667 cites W2136192914 @default.
- W2013357667 cites W2145919777 @default.
- W2013357667 cites W2162279862 @default.
- W2013357667 cites W2162924518 @default.
- W2013357667 doi "https://doi.org/10.1016/s0006-3495(94)80548-x" @default.
- W2013357667 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1225430" @default.
- W2013357667 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7948700" @default.
- W2013357667 hasPublicationYear "1994" @default.
- W2013357667 type Work @default.
- W2013357667 sameAs 2013357667 @default.
- W2013357667 citedByCount "14" @default.
- W2013357667 countsByYear W20133576672013 @default.
- W2013357667 crossrefType "journal-article" @default.
- W2013357667 hasAuthorship W2013357667A5002137973 @default.
- W2013357667 hasAuthorship W2013357667A5028243612 @default.
- W2013357667 hasAuthorship W2013357667A5031049857 @default.
- W2013357667 hasAuthorship W2013357667A5034691817 @default.
- W2013357667 hasAuthorship W2013357667A5066073351 @default.
- W2013357667 hasBestOaLocation W20133576671 @default.
- W2013357667 hasConcept C113196181 @default.
- W2013357667 hasConcept C120665830 @default.
- W2013357667 hasConcept C121332964 @default.
- W2013357667 hasConcept C121745418 @default.
- W2013357667 hasConcept C178790620 @default.
- W2013357667 hasConcept C181199279 @default.
- W2013357667 hasConcept C185592680 @default.
- W2013357667 hasConcept C192527728 @default.
- W2013357667 hasConcept C2776706248 @default.
- W2013357667 hasConcept C2779811187 @default.
- W2013357667 hasConcept C515207424 @default.
- W2013357667 hasConcept C520434653 @default.
- W2013357667 hasConcept C55005982 @default.
- W2013357667 hasConcept C55493867 @default.
- W2013357667 hasConcept C71240020 @default.
- W2013357667 hasConcept C8010536 @default.
- W2013357667 hasConcept C91881484 @default.
- W2013357667 hasConceptScore W2013357667C113196181 @default.
- W2013357667 hasConceptScore W2013357667C120665830 @default.
- W2013357667 hasConceptScore W2013357667C121332964 @default.
- W2013357667 hasConceptScore W2013357667C121745418 @default.
- W2013357667 hasConceptScore W2013357667C178790620 @default.
- W2013357667 hasConceptScore W2013357667C181199279 @default.
- W2013357667 hasConceptScore W2013357667C185592680 @default.
- W2013357667 hasConceptScore W2013357667C192527728 @default.
- W2013357667 hasConceptScore W2013357667C2776706248 @default.
- W2013357667 hasConceptScore W2013357667C2779811187 @default.
- W2013357667 hasConceptScore W2013357667C515207424 @default.
- W2013357667 hasConceptScore W2013357667C520434653 @default.
- W2013357667 hasConceptScore W2013357667C55005982 @default.
- W2013357667 hasConceptScore W2013357667C55493867 @default.
- W2013357667 hasConceptScore W2013357667C71240020 @default.
- W2013357667 hasConceptScore W2013357667C8010536 @default.
- W2013357667 hasConceptScore W2013357667C91881484 @default.
- W2013357667 hasIssue "2" @default.
- W2013357667 hasLocation W20133576671 @default.
- W2013357667 hasLocation W20133576672 @default.
- W2013357667 hasLocation W20133576673 @default.
- W2013357667 hasLocation W20133576674 @default.
- W2013357667 hasOpenAccess W2013357667 @default.
- W2013357667 hasPrimaryLocation W20133576671 @default.
- W2013357667 hasRelatedWork W1994239116 @default.
- W2013357667 hasRelatedWork W2013357667 @default.
- W2013357667 hasRelatedWork W2049510987 @default.
- W2013357667 hasRelatedWork W2054181767 @default.
- W2013357667 hasRelatedWork W2070198970 @default.
- W2013357667 hasRelatedWork W2086572669 @default.
- W2013357667 hasRelatedWork W2149502868 @default.
- W2013357667 hasRelatedWork W2521273224 @default.
- W2013357667 hasRelatedWork W2893430273 @default.
- W2013357667 hasRelatedWork W2949091892 @default.
- W2013357667 hasVolume "67" @default.
- W2013357667 isParatext "false" @default.
- W2013357667 isRetracted "false" @default.
- W2013357667 magId "2013357667" @default.