Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013377060> ?p ?o ?g. }
- W2013377060 endingPage "71" @default.
- W2013377060 startingPage "65" @default.
- W2013377060 abstract "The effects of various environmental conditions and chemical compounds on the activity and stability of the lipolytic preparation obtained from a wild strain of Penicillium aurantiogriseum were characterized during a preliminary evaluation of its potential for use in biocatalysis. In aqueous solution, the optimum pH for activity was 8.0 and the enzyme was stable between pH 6.0 and pH 9.0. In assays of 1 min duration carried out at pH 8.0, enzyme activities were quite high from 37 to 70 °C, with a maximum at 60 °C. However, thermal stability was rather low at temperatures higher than 28 °C. Hydrolytic activity was enhanced by Mg2+, Zn2+, Co2+ and Mn2+, but was inhibited by Cu2+, Ba2+ and Hg2+, while Ca2+ had no effect. Sodium azide activated the enzyme. Triton X-100 caused an inhibition of 52%, while Tween 80 and SDS had negligible effects on enzymatic activity. Despite the low ratio of the activity towards p-nitrophenyl palmitate (pNPP) in organic medium to that in aqueous medium (RO/A=4.3×10−2), the enzyme showed a good stability in organic solvents with high log P values, the best result being in n-heptane (114% residual activity). These promising results with the crude preparation justify the undertaking of purification studies and the use of the pure enzyme in a more in-depth investigation for its potential in biocatalysis in organic solvents." @default.
- W2013377060 created "2016-06-24" @default.
- W2013377060 creator A5003570435 @default.
- W2013377060 creator A5018676300 @default.
- W2013377060 creator A5040229377 @default.
- W2013377060 creator A5057455409 @default.
- W2013377060 date "2004-04-01" @default.
- W2013377060 modified "2023-09-29" @default.
- W2013377060 title "Activity and stability of a crude lipase from Penicillium aurantiogriseum in aqueous media and organic solvents" @default.
- W2013377060 cites W1175056953 @default.
- W2013377060 cites W1563222268 @default.
- W2013377060 cites W1854079265 @default.
- W2013377060 cites W1965208113 @default.
- W2013377060 cites W1967422084 @default.
- W2013377060 cites W1981508058 @default.
- W2013377060 cites W1993689943 @default.
- W2013377060 cites W1995102275 @default.
- W2013377060 cites W1999878208 @default.
- W2013377060 cites W2005019767 @default.
- W2013377060 cites W2019360071 @default.
- W2013377060 cites W2021724204 @default.
- W2013377060 cites W2034343666 @default.
- W2013377060 cites W2037347043 @default.
- W2013377060 cites W2044733318 @default.
- W2013377060 cites W2046763790 @default.
- W2013377060 cites W2048023007 @default.
- W2013377060 cites W2055920875 @default.
- W2013377060 cites W2062746330 @default.
- W2013377060 cites W2063586688 @default.
- W2013377060 cites W2070129821 @default.
- W2013377060 cites W2085347485 @default.
- W2013377060 cites W2091985339 @default.
- W2013377060 cites W2093342804 @default.
- W2013377060 cites W2109194627 @default.
- W2013377060 cites W2118383750 @default.
- W2013377060 cites W2126130975 @default.
- W2013377060 cites W2126471872 @default.
- W2013377060 cites W2312266089 @default.
- W2013377060 cites W4293247451 @default.
- W2013377060 cites W4299141148 @default.
- W2013377060 doi "https://doi.org/10.1016/s1369-703x(03)00165-7" @default.
- W2013377060 hasPublicationYear "2004" @default.
- W2013377060 type Work @default.
- W2013377060 sameAs 2013377060 @default.
- W2013377060 citedByCount "131" @default.
- W2013377060 countsByYear W20133770602012 @default.
- W2013377060 countsByYear W20133770602013 @default.
- W2013377060 countsByYear W20133770602014 @default.
- W2013377060 countsByYear W20133770602015 @default.
- W2013377060 countsByYear W20133770602016 @default.
- W2013377060 countsByYear W20133770602017 @default.
- W2013377060 countsByYear W20133770602018 @default.
- W2013377060 countsByYear W20133770602019 @default.
- W2013377060 countsByYear W20133770602020 @default.
- W2013377060 countsByYear W20133770602021 @default.
- W2013377060 countsByYear W20133770602022 @default.
- W2013377060 countsByYear W20133770602023 @default.
- W2013377060 crossrefType "journal-article" @default.
- W2013377060 hasAuthorship W2013377060A5003570435 @default.
- W2013377060 hasAuthorship W2013377060A5018676300 @default.
- W2013377060 hasAuthorship W2013377060A5040229377 @default.
- W2013377060 hasAuthorship W2013377060A5057455409 @default.
- W2013377060 hasConcept C161790260 @default.
- W2013377060 hasConcept C178790620 @default.
- W2013377060 hasConcept C181199279 @default.
- W2013377060 hasConcept C184651966 @default.
- W2013377060 hasConcept C185592680 @default.
- W2013377060 hasConcept C191503008 @default.
- W2013377060 hasConcept C2779697368 @default.
- W2013377060 hasConcept C2780786110 @default.
- W2013377060 hasConcept C2781417787 @default.
- W2013377060 hasConcept C28212737 @default.
- W2013377060 hasConcept C33633552 @default.
- W2013377060 hasConcept C43617362 @default.
- W2013377060 hasConcept C59061564 @default.
- W2013377060 hasConcept C65024703 @default.
- W2013377060 hasConcept C94412978 @default.
- W2013377060 hasConceptScore W2013377060C161790260 @default.
- W2013377060 hasConceptScore W2013377060C178790620 @default.
- W2013377060 hasConceptScore W2013377060C181199279 @default.
- W2013377060 hasConceptScore W2013377060C184651966 @default.
- W2013377060 hasConceptScore W2013377060C185592680 @default.
- W2013377060 hasConceptScore W2013377060C191503008 @default.
- W2013377060 hasConceptScore W2013377060C2779697368 @default.
- W2013377060 hasConceptScore W2013377060C2780786110 @default.
- W2013377060 hasConceptScore W2013377060C2781417787 @default.
- W2013377060 hasConceptScore W2013377060C28212737 @default.
- W2013377060 hasConceptScore W2013377060C33633552 @default.
- W2013377060 hasConceptScore W2013377060C43617362 @default.
- W2013377060 hasConceptScore W2013377060C59061564 @default.
- W2013377060 hasConceptScore W2013377060C65024703 @default.
- W2013377060 hasConceptScore W2013377060C94412978 @default.
- W2013377060 hasIssue "1" @default.
- W2013377060 hasLocation W20133770601 @default.
- W2013377060 hasOpenAccess W2013377060 @default.
- W2013377060 hasPrimaryLocation W20133770601 @default.
- W2013377060 hasRelatedWork W1971222377 @default.
- W2013377060 hasRelatedWork W2015263265 @default.