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- W2013402829 abstract "Nonlinear optical phase conjugation by degenerate four-wave mixing is demonstrated as a sensitive absorbance detection method for microbore high-performance liquid chromatography. An argon ion laser operating at the 488-nm line is used as the excitation light source to generate the wave-mixing signal for dabsyl-labeled amino acids. Advantages of the nonlinear laser detection method include: virtually 100% optical signal collection efficiency, generation of the signal in the form of a coherent laser beam, signal measurement against a virtually dark background, reliable detection of small absorbance values, excellent detection sensitivity for both fluorescing and non-fluorescing analytes, relatively simple one-color one-laser optical setup, and low power or energy requirements for continuous-wave or pulsed lasers. Using our one-laser one-color nonlinear laser detector for absorbance measurements in liquid chromatography, we report a crude preliminary injected detection limit of 780 fmol for glycine." @default.
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- W2013402829 date "1998-05-01" @default.
- W2013402829 modified "2023-10-16" @default.
- W2013402829 title "Absorbance detection of amino acids by laser wave mixing in microbore liquid chromatography" @default.
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- W2013402829 doi "https://doi.org/10.1016/s0021-9673(98)00022-3" @default.
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