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- W2013414489 abstract "Using bromodeoxyuridine (BrdU) and an anti-BrdU monoclonal antibody (Mab), the fraction of cells in S phase of the Y79 human retinoblastoma line maintained in an organ culture system was determined. The average labeling index (LI) (S phase cell fraction) was 21.5% after 2–15 days in vitro. The effect of butyric acid, which has been reported by others to inhibit the growth of Y79 retinoblastoma cells, was then evaluated in a similar in vitro system. Following treatment with 2 and 3 mM of butyric acid, extensive necrosis was seen in the cultures, and the LI of the surviving cells was significantly (p < 0.01) lower (4.1 and 2.9%, respectively) than that of the control cultures (21.1%). Forty-eight hours after removal of butyric acid, the LI of the cultures treated respectively with 2 and 3 mM of butyric acid returned to the control level. Our findings suggest that the effect of butyric acid on the cell kinetics of Y79 retinoblastoma cells may be a reversible blocking effect on the G1 phase of the cell cycle." @default.
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- W2013414489 date "1987-01-01" @default.
- W2013414489 modified "2023-09-27" @default.
- W2013414489 title "Determination by Anti-Bromodeoxyuridine Monoclonal Antibody of Cells in S Phase in the Human Y79 Retinoblastoma Line Maintained in an Organ Culture System: Effects of Butyric Acid" @default.
- W2013414489 doi "https://doi.org/10.1159/000265495" @default.
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