FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013422859> ?p ?o ?g. }

• W2013422859 endingPage "284" @default.
• W2013422859 startingPage "273" @default.
• W2013422859 abstract "Electroporation creates transient pores in the plasma membrane to introduce macromolecules within a cell or cell population. Generally, electrical pulses are delivered between two electrodes separated from each other, making electroporation less likely to be localised. We have developed a new device combining local pressure ejection with local electroporation through a double-barrelled glass micropipette to transfer impermeable macromolecules in brain slices or in cultured HEK293 cells. The design achieves better targeting of the site of pressure ejection with that of electroporation. With this technique, we have been able to limit the delivery of propidium iodide or dextran amine within areas of 100-200 micrometer. We confirm that local electroporation is transient and show that when combined with pressure ejection, it allows local transfection of EGFP plasmids within HEK293 cells or within cerebellar and hippocampal slice cultures. We further show that local electroporation is less damaging when compared to global electroporation using two separate electrodes. Focal delivery of dextran amine dyes within trapezoid body fibres allowed tracing axonal tracts within brainstem slices, enabling the study of identified calyx of Held presynaptic terminals in living brain tissue. This labelling method can be used to target small nuclei in neuronal tissue and is generally applicable to the study of functional synaptic connectivity, or live axonal tracing in a variety of brain areas." @default.
• W2013422859 created "2016-06-24" @default.
• W2013422859 creator A5009468044 @default.
• W2013422859 creator A5018827554 @default.
• W2013422859 creator A5056759223 @default.
• W2013422859 date "2009-03-01" @default.
• W2013422859 modified "2023-09-25" @default.
• W2013422859 title "Focal macromolecule delivery in neuronal tissue using simultaneous pressure ejection and local electroporation" @default.
• W2013422859 cites W1487546036 @default.
• W2013422859 cites W1877594537 @default.
• W2013422859 cites W1904722209 @default.
• W2013422859 cites W1969120034 @default.
• W2013422859 cites W1971647703 @default.
• W2013422859 cites W1974297452 @default.
• W2013422859 cites W1979897011 @default.
• W2013422859 cites W1994655859 @default.
• W2013422859 cites W2004085085 @default.
• W2013422859 cites W2006069125 @default.
• W2013422859 cites W2008800998 @default.
• W2013422859 cites W2016016372 @default.
• W2013422859 cites W2043289005 @default.
• W2013422859 cites W2043297269 @default.
• W2013422859 cites W2047905524 @default.
• W2013422859 cites W2059039373 @default.
• W2013422859 cites W2060289254 @default.
• W2013422859 cites W2068196810 @default.
• W2013422859 cites W2070121678 @default.
• W2013422859 cites W2076378288 @default.
• W2013422859 cites W2076415281 @default.
• W2013422859 cites W2081518046 @default.
• W2013422859 cites W2088973746 @default.
• W2013422859 cites W2097653641 @default.
• W2013422859 cites W2100585525 @default.
• W2013422859 cites W2140031668 @default.
• W2013422859 cites W2145062003 @default.
• W2013422859 cites W2162831063 @default.
• W2013422859 cites W3024831056 @default.
• W2013422859 cites W41560684 @default.
• W2013422859 doi "https://doi.org/10.1016/j.jneumeth.2008.10.021" @default.
• W2013422859 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2680959" @default.
• W2013422859 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19014970" @default.
• W2013422859 hasPublicationYear "2009" @default.
• W2013422859 type Work @default.
• W2013422859 sameAs 2013422859 @default.
• W2013422859 citedByCount "13" @default.
• W2013422859 countsByYear W20134228592012 @default.
• W2013422859 countsByYear W20134228592014 @default.
• W2013422859 countsByYear W20134228592016 @default.
• W2013422859 countsByYear W20134228592017 @default.
• W2013422859 countsByYear W20134228592018 @default.
• W2013422859 countsByYear W20134228592019 @default.
• W2013422859 crossrefType "journal-article" @default.
• W2013422859 hasAuthorship W2013422859A5009468044 @default.
• W2013422859 hasAuthorship W2013422859A5018827554 @default.
• W2013422859 hasAuthorship W2013422859A5056759223 @default.
• W2013422859 hasBestOaLocation W20134228591 @default.
• W2013422859 hasConcept C104317684 @default.
• W2013422859 hasConcept C12554922 @default.
• W2013422859 hasConcept C144024400 @default.
• W2013422859 hasConcept C149923435 @default.
• W2013422859 hasConcept C185592680 @default.
• W2013422859 hasConcept C186852380 @default.
• W2013422859 hasConcept C190283241 @default.
• W2013422859 hasConcept C2775934118 @default.
• W2013422859 hasConcept C2777368234 @default.
• W2013422859 hasConcept C2779530196 @default.
• W2013422859 hasConcept C2908647359 @default.
• W2013422859 hasConcept C31573885 @default.
• W2013422859 hasConcept C55493867 @default.
• W2013422859 hasConcept C86803240 @default.
• W2013422859 hasConcept C95444343 @default.
• W2013422859 hasConceptScore W2013422859C104317684 @default.
• W2013422859 hasConceptScore W2013422859C12554922 @default.
• W2013422859 hasConceptScore W2013422859C144024400 @default.
• W2013422859 hasConceptScore W2013422859C149923435 @default.
• W2013422859 hasConceptScore W2013422859C185592680 @default.
• W2013422859 hasConceptScore W2013422859C186852380 @default.
• W2013422859 hasConceptScore W2013422859C190283241 @default.
• W2013422859 hasConceptScore W2013422859C2775934118 @default.
• W2013422859 hasConceptScore W2013422859C2777368234 @default.
• W2013422859 hasConceptScore W2013422859C2779530196 @default.
• W2013422859 hasConceptScore W2013422859C2908647359 @default.
• W2013422859 hasConceptScore W2013422859C31573885 @default.
• W2013422859 hasConceptScore W2013422859C55493867 @default.
• W2013422859 hasConceptScore W2013422859C86803240 @default.
• W2013422859 hasConceptScore W2013422859C95444343 @default.
• W2013422859 hasIssue "2" @default.
• W2013422859 hasLocation W20134228591 @default.
• W2013422859 hasLocation W20134228592 @default.
• W2013422859 hasLocation W20134228593 @default.
• W2013422859 hasLocation W20134228594 @default.
• W2013422859 hasLocation W20134228595 @default.
• W2013422859 hasOpenAccess W2013422859 @default.
• W2013422859 hasPrimaryLocation W20134228591 @default.
• W2013422859 hasRelatedWork W17358665 @default.
• W2013422859 hasRelatedWork W1966253856 @default.
• W2013422859 hasRelatedWork W2008800998 @default.
• W2013422859 hasRelatedWork W2051719328 @default.

• next