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• W2013430266 abstract "Abstract The fungus Colletotrichum capsici was isolated from the diseased fruits of Chilli plant, Capsicum annuum . The isolated test fungus was identified by its morphological and molecular characteristic features. For the first time, the fungus was screened for the production of taxol on modified liquid medium. The presence of taxol was confirmed by the spectroscopic and chromatographic methods of analyses. The amount of taxol produced by this fungus was quantified by HPLC. The maximum amount of fungal taxol production was recorded as 687 μg/L. The production rate was 13 740‐fold higher than that, previously reported for the fungus Taxomyces andreanae . The extracted fungal taxol showed a strong cytotoxic activity in an in vitro culture of human cancer cells indicating that the increase in taxol concentration induces increased cell death. A PCR‐based screening for taxadiene synthase ( ts ), a unique gene in the formation of the taxane skeleton, confirmed the molecular blueprint for taxol biosynthesis. The results show that the fungus C. capsici is an excellent candidate for an alternate source of taxol supply and can serve as a potential species for genetic engineering to enhance the production of taxol to a higher level." @default.
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• W2013430266 date "2011-02-02" @default.
• W2013430266 modified "2023-10-18" @default.
• W2013430266 title "In vitro screening of taxol, an anticancer drug produced by the fungus, Colletotrichum capsici" @default.
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• W2013430266 doi "https://doi.org/10.1002/elsc.201000119" @default.
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