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- W2013454314 abstract "In their recent letter ‘Protein interactions: is seeing believing?’ [1], Mackay and colleagues discuss sources of artefacts intrinsic to experimental techniques that are commonly used to gather evidence of protein interaction. More specifically, they point out that co-immunoprecipitation and pull-down experiments are often taken as evidence of direct protein interaction, whereas these in vivo observations are seldom corroborated by more direct biophysical approaches. Although, we agree with most of their analysis, we fear that readers might be led to the conclusion that the bulk of the experiments annotated in protein-interaction databases such as MINT (the Molecular INTeraction database) [2] do not provide much evidence of functional contact between the two partner proteins, and they could assume that the information is too preliminary for drawing biological conclusions about the local or global properties of protein-interaction networks." @default.
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- W2013454314 date "2008-06-01" @default.
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- W2013454314 title "Protein interactions: integration leads to belief" @default.
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- W2013454314 doi "https://doi.org/10.1016/j.tibs.2008.04.002" @default.
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