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• W2013455871 endingPage "1909" @default.
• W2013455871 startingPage "1900" @default.
• W2013455871 abstract "ABSTRACT Sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:NM (nonmotile) is a unique clone that causes outbreaks of hemorrhagic colitis and hemolytic-uremic syndrome. In well-defined clusters of cases, we have observed significant variability in pulsed-field gel electrophoresis (PFGE) patterns which could indicate coinfection by different strains. An analysis of randomly selected progeny colonies of an outbreak strain after subcultivation demonstrated that they displayed either the cognate PFGE outbreak pattern or one of four additional patterns and were <89% similar. These profound alterations were associated with changes in the genomic position of one of two Shiga toxin 2-encoding genes ( stx 2 ) in the outbreak strain or with the loss of this gene. The two stx 2 alleles in the outbreak strain were identical but were flanked with phage-related sequences with only 77% sequence identity. Neither of these phages produced plaques, but one lysogenized E. coli K-12 and integrated in yecE in the lysogens and the wild-type strain. The presence of two stx 2 genes which correlated with increased production of Stx2 in vitro but not with the clinical outcome of infection was also found in 14 (21%) of 67 SF EHEC O157:NM isolates from sporadic cases of human disease. The variability of PFGE patterns for the progeny of a single colony must be considered when interpreting PFGE patterns in SF EHEC O157-associated outbreaks." @default.
• W2013455871 created "2016-06-24" @default.
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• W2013455871 date "2006-03-01" @default.
• W2013455871 modified "2023-09-27" @default.
• W2013455871 title "Chromosomal Dynamism in Progeny of Outbreak-Related Sorbitol-Fermenting Enterohemorrhagic<i>Escherichia coli</i>O157:NM" @default.
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• W2013455871 doi "https://doi.org/10.1128/aem.72.3.1900-1909.2006" @default.
• W2013455871 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1393231" @default.
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