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• W2013476869 abstract "The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer." @default.
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• W2013476869 date "2002-08-23" @default.
• W2013476869 modified "2023-10-17" @default.
• W2013476869 title "Protease-Catalyzed Peptide Synthesis on Solid Support" @default.
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• W2013476869 doi "https://doi.org/10.1021/ja026912d" @default.
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