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• W2013499585 abstract "Brucella are resistant to polymyxin B (PB), but their relative susceptibility to PB and its derivative, colistin (COL) has not been rigorously or systematically studied. Comparative susceptibility of Brucella reference strains, vaccine strain RB51, and Brucella isolates from marine mammals to these two cationic peptides were determined by Etest. Vast differences among Brucella species were found in susceptibility to both PB and COL. Brucella demonstrated similar pattern of relative susceptibility to PB as that of COL, but they were less susceptible to COL. Both B. melitensis and B. suis were the least susceptible to polymyxins and rough strains were more susceptible to both PB and COL than the smooth except for the BvrR mutant. Strains were generally less susceptible to PB when cultured in CO2 rather than ambient air; some became more susceptible in acidified medium. Results show that environment cultural conditions must be considered when selecting for CO2-independent strains of Brucella especially the vaccine strain RB51 on selective media containing PB. Our observations extend basic knowledge of the differential resistance of Brucella to polymyxins. Brucella sont résistants à polymyxin B (PB), mais leur susceptibilité relative à PB son dérivé, colistin (COL) n’a pas été rigoureusement ou systématiquement étudiée. Susceptibilité comparative de Brucella référence contraintes, la contrainte vaccinique RB51, et Brucella isolats à partir mammifères marins à ces deux peptides cationiques ont été déterminés par Etest. Vastes différences parmi Brucella espèces ont été trouvées dans susceptibilité au PB. Brucella a démontré le modèle similaire de susceptibilité relative à COL mais était moins susceptible. Tous deux B. melitensis et B. suis étaient les moindres susceptibles et les contraintes approximatives étaient plus susceptibles que lisses excepté un mutant de BvrR. Les contraintes étaient généralement moins susceptibles à PB une fois cultivées dans la CO2 plutôt qu’ air ambiant; certains sont devenus plus susceptibles dans le milieu acidifié. Résultats montrent que l’environnement conditions culturelles doit être considéré en choisissant pour la CO2 indépendantes de – de Brucella particulièrement vaccinique RB51 sur des médias sélectifs contenant des polymyxins. Nos observations prolongent la connaissance de base de la résistance différentielle de Brucella à PB." @default.
• W2013499585 created "2016-06-24" @default.
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• W2013499585 date "2010-03-01" @default.
• W2013499585 modified "2023-09-25" @default.
• W2013499585 title "Effect of polymyxin B and environmental conditions on isolation of Brucella species and the vaccine strain RB51" @default.
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• W2013499585 doi "https://doi.org/10.1016/j.cimid.2008.08.005" @default.
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