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• W2013543937 abstract "Electron-spin resonance-spin trapping has been used to detect lipid-derived radicals in liposomes. Using the lipid-soluble spin trap 2-methyl-nitrosopropane (MNP), we have detected both the lipid and hydrogen-atom spin adducts in liposomes composed of a fully saturated phospholipid (dimyristoylphosphatidylcholine, DMPC), with various mol fractions of unsaturated phospholipid (1-palmitoyl-2-arachidonoylphosphatidylcholine, PAPC) or fatty acid (arachidonic acid, AA). The lipid-derived spin adduct formed during autoxidation of liposomes was separated by thin-layer chromatography and found to co-migrate with the product(s) formed by direct addition of MNP to the corresponding unsaturated lipid or fatty acid. Both the MNP-PAPC and MNP-AA spin adducts showed some restriction of rotational motion when in the liposome bilayer (rotational correlation times 0.72 and 0.69·10−9 s, respectively), and nitrogen hyperfine coupling constants (14.94–14.96 G) consistent with a hydrophobic localization. Radical versus non-radical mechanisms of spin adduct formation during liposome autoxidation were separated using α-tocopherol as a radical scavenger. The utility of nitroso spin traps in trapping of radicals in liposomes is discussed." @default.
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• W2013543937 creator A5024996281 @default.
• W2013543937 creator A5035372773 @default.
• W2013543937 date "1989-08-01" @default.
• W2013543937 modified "2023-09-25" @default.
• W2013543937 title "Spin trapping of lipid-derived radicals in liposomes" @default.
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• W2013543937 doi "https://doi.org/10.1016/0304-4165(89)90015-9" @default.
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