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• W2013596747 abstract "Adherent A431, BHK-21, and C2C12 cells were cultured on a flexible interface formed between two immiscible liquid phases: (i) hydrophobic perfluorodecalin (PFD) and (ii) aqueous culture medium (DMEM). BHK-21 cells formed multicellular aggregates characterized by irregular shapes. A431, as well as C2C12 cells, grew as tight multicellular sheets of 3-D cells. Enhanced mass transfer and facilitated access of the cells to the O2 dissolved in PFD/DMEM by approx. 250 % and thereby increased the density of BHK-21 cells. Thus the liquid/liquid system is a simple, ready-to-use, and fully scalable (independent of vessel shapes); consequently it is a method for 3-D cultures of adherent animal cells in which the growth of anchorage-dependent cells is not limited by confluence effect." @default.
• W2013596747 created "2016-06-24" @default.
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• W2013596747 date "2013-05-12" @default.
• W2013596747 modified "2023-09-25" @default.
• W2013596747 title "Morphology and growth of mammalian cells in a liquid/liquid culture system supported with oxygenated perfluorodecalin" @default.
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• W2013596747 doi "https://doi.org/10.1007/s10529-013-1218-2" @default.
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