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- W2013642507 abstract "Introduction: The small heat shock protein (HSP27, currently referred to as HSPB1) has been implicated in regulating vascular smooth muscle tone. HSPB1 is phosphorylated by a stress activated signaling cascade which includes p38 MAP kinase and MAPKAP kinase II (MK2). The purpose of this investigation was to determine if HSPB1 expression correlated with vascular responses in human tissue. Methods: Discarded segments of human saphenous veins (HSV) were obtained after peripheral and coronary revascularizations. Physiologic responses were tested in a muscle bath and immunoblotting was performed to determine HSPB1 expression. A recombinant fusion protein was engineered to contain a cell penetrating peptide sequence and HSPB1 (CPP-HSPB1). Results: 40% of veins harvested for aortocoronary bypass had no functional viability (no contractile response to KCl 110 mM, n=39). In functionally viable HSV, HSPB1 expression and phosphorylation were inversely associated with relaxation in response to sodium nitroprusside (SNP, 10−7 M, Panel A). Inhibiting HSP27 phosphorylation by pretreatment with SB203580 (25 μM, a p38 MAPK inhibitor, Panel B diamond), or a cell permeant peptide inhibitor of MK2 (30 μM, Panel B, open circle), led to enhanced SNP (10−7 M) induced relaxation of HSV (n=3, p <0.05 for both treatments). Treatment of rat aortic smooth muscle (which contains low levels of HSPB1) with CPP-HSPB1 led increased amounts of HSPB1 and was associated with decreased relaxation responses SNP (10−7 M, n=3, p<0.05, Panel C). Conclusions: Taken together, these studies support a role for the stress protein, HSPB1, in impaired relaxation of smooth muscle. Therapies directed at decreasing the phosphorylation/expression of HSPB1 may ameliorate vasospasm in HSV." @default.
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- W2013642507 date "2010-02-01" @default.
- W2013642507 modified "2023-10-18" @default.
- W2013642507 title "Expression of the Heat Shock Protein, HSPB1, Is Associated with Impaired Smooth Muscle Relaxation" @default.
- W2013642507 doi "https://doi.org/10.1016/j.jss.2009.11.523" @default.
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