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- W2013646481 abstract "Abstract Enzyme I (EI) of the bacterial phosphoenolpyruvate:glycose phosphotransferase system (PTS) is autocatalytically phosphorylated by P-enolpyruvate. We report here an ATP-dependent kinase (EI-K) from Escherichia coli that reversibly phosphorylates EI at its active site histidine; ATP and EI-K can therefore replace P-enolpyruvate. EI-K contains a bound cofactor that is lost during purification with concomitant loss of activity. NAD+ and NADP+ substitute for the cofactor and restore activity to the apoenzyme, whereas their analogues are inactive. The pyridine nucleotides do not activate EI-K by covalent modification (e.g. ADP-ribosylation), but must be present during the kinase reaction. NADH and NADPH are potent inhibitors of EI-K at all stages of purity, and enzyme activity in a mixture of NAD+ and NADH depends on the ratio of the two pyridine nucleotides. Inhibition is observed with reduced β-NMN and α-NADH, but neither is as effective as β-NADH. The reverse reaction, the transfer of the phosphoryl moiety from phospho-EI to ADP, also requires NAD+ or NADP+. In the absence of NAD+ or NADH, [32P]phospho-EI is hydrolyzed to 32Pi, suggesting that EI-K can act as a phospho-EI phosphatase. EI kinase may serve as a link between PTS-driven sugar transport and the electron transport chain." @default.
- W2013646481 created "2016-06-24" @default.
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- W2013646481 date "1996-06-01" @default.
- W2013646481 modified "2023-09-27" @default.
- W2013646481 title "Active Site Phosphorylation of Enzyme I of the Bacterial Phosphotransferase System by an ATP-dependent Kinase" @default.
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- W2013646481 doi "https://doi.org/10.1074/jbc.271.25.15285" @default.
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