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• W2013669679 abstract "The restriction of cell-free HIV-1 infection has been demonstrated in placental trophoblast choriocarcinoma BeWo cells. We tried to determine the level of the viral replication cycle at which this restriction occurs. BeWo cells produce infectious viruses after transfection with HIV-1 plasmids, independently of viral tropism. CCR5 and CXCR4, but not the CD4 molecule, were detected at the cell surface. We therefore derived CD4-expressing clones from transfected BeWo cells. Cell-free virus infection of these clones resulted in neither virus production nor viral sequence integration, indicating that the restriction occurs before integration of the virus. If we used luciferase reporter viruses pseudotyped with HIV-1 Env R5 and X4 for infection, no luciferase activity was detected, even in the BeWo-CD4+ clone, in contrast to what was observed in VSV-G pseudotyped virus infection. Our results show that infection of trophoblast-derived cells with cell-free virus is at least restricted at the level of entry. Thus, BeWo is an interesting human placental cell line that is resistant to HIV-1, even if CD4, CXCR4, and CCR5 are expressed." @default.
• W2013669679 created "2016-06-24" @default.
• W2013669679 creator A5021504349 @default.
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• W2013669679 creator A5068589474 @default.
• W2013669679 date "2003-10-01" @default.
• W2013669679 modified "2023-10-14" @default.
• W2013669679 title "Cell-Free HIV Type 1 Infection is Restricted in the Human Trophoblast Choriocarcinoma BeWo Cell Line, Even with Expression of CD4, CXCR4 and CCR5" @default.
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• W2013669679 doi "https://doi.org/10.1089/088922203322493021" @default.
• W2013669679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14585217" @default.
• W2013669679 hasPublicationYear "2003" @default.
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