Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013678635> ?p ?o ?g. }
- W2013678635 endingPage "29" @default.
- W2013678635 startingPage "22" @default.
- W2013678635 abstract "Insulin receptor activities, i.e., insulin binding and tyrosine kinase activation depend on the lipid environment of the receptor. As detergent may disrupt or interfere with this environment, we investigated the effect of various common detergents on insulin receptor properties. Experiments were carried out (i) on solubilized and partially purified insulin receptor and (ii) on the receptor reconstituted into phosphatidylcholine vesicles. The detergents tested, Triton X-100, octyl-beta-D-glucopyranoside, octyl-beta-D-thioglucopyranoside, 3[(3-cholamidopropyl)dimethylammonio]propanesulfonic acid (Chaps), and Na deoxycholate affected the insulin receptor properties differently when compared with the control receptor in the absence of detergent. On the partially purified insulin receptor, Na deoxycholate inhibited both insulin receptor activities; octyl-beta-D-glucopyranoside and octyl-beta-D-thioglucopyranoside decreased insulin binding and kinase activation as their concentration increased, particularly above their respective critical micellar concentration (CMC). Triton X-100 was the only detergent which allowed an increase of insulin binding and kinase activation throughout the whole range of concentrations assayed. Reconstitution of the receptor into phosphatidylcholine vesicles protected the receptor from the direct effects of the detergents, for both the stimulation observed with Triton X-100 and the inhibition produced by the other detergents. In order to determine the effect of detergents on the oligomeric forms of the soluble insulin receptor, we investigated a new rapid sucrose gradient centrifugation technique. Insulin receptors were detected on the gradient by 125I insulin binding. For low concentrations of detergent, i.e., near the CMC, octylglucoside, Chaps, and Triton X-100 favored the (alpha 2 beta 2)2 oligomeric form of the receptor. Higher concentrations of Triton X-100 did not modify the polymeric state of the receptor. In contrast, octylglucoside and Chaps induced an increase in the sedimentation coefficient of the receptor which appeared as (alpha 2 beta 2)3 and (alpha 2 beta 2)4 forms. These alterations in the oligomerization status of the insulin receptor may explain the deleterious effects observed with both Chaps and octylglucoside at higher concentrations." @default.
- W2013678635 created "2016-06-24" @default.
- W2013678635 creator A5001901825 @default.
- W2013678635 creator A5013347953 @default.
- W2013678635 creator A5014681063 @default.
- W2013678635 creator A5076966946 @default.
- W2013678635 date "1992-04-01" @default.
- W2013678635 modified "2023-10-18" @default.
- W2013678635 title "Detergents affect insulin binding, tyrosine kinase activity and oligomeric structure of partially purified insulin receptors" @default.
- W2013678635 cites W1479900350 @default.
- W2013678635 cites W1483900477 @default.
- W2013678635 cites W1503737286 @default.
- W2013678635 cites W1527803305 @default.
- W2013678635 cites W1563765887 @default.
- W2013678635 cites W1965371391 @default.
- W2013678635 cites W1970923910 @default.
- W2013678635 cites W1978304038 @default.
- W2013678635 cites W1991710558 @default.
- W2013678635 cites W1994837128 @default.
- W2013678635 cites W1996827534 @default.
- W2013678635 cites W2000438275 @default.
- W2013678635 cites W2015000698 @default.
- W2013678635 cites W2016657349 @default.
- W2013678635 cites W2043593761 @default.
- W2013678635 cites W2043813981 @default.
- W2013678635 cites W205031339 @default.
- W2013678635 cites W2052596625 @default.
- W2013678635 cites W2064070437 @default.
- W2013678635 cites W2067451034 @default.
- W2013678635 cites W2068994489 @default.
- W2013678635 cites W2071288198 @default.
- W2013678635 cites W2072591785 @default.
- W2013678635 cites W2085979165 @default.
- W2013678635 cites W2086075785 @default.
- W2013678635 cites W2086560292 @default.
- W2013678635 cites W2100837269 @default.
- W2013678635 cites W2143444693 @default.
- W2013678635 cites W32049552 @default.
- W2013678635 cites W4293247451 @default.
- W2013678635 doi "https://doi.org/10.1016/0003-9861(92)90131-f" @default.
- W2013678635 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1312805" @default.
- W2013678635 hasPublicationYear "1992" @default.
- W2013678635 type Work @default.
- W2013678635 sameAs 2013678635 @default.
- W2013678635 citedByCount "11" @default.
- W2013678635 countsByYear W20136786352018 @default.
- W2013678635 countsByYear W20136786352019 @default.
- W2013678635 crossrefType "journal-article" @default.
- W2013678635 hasAuthorship W2013678635A5001901825 @default.
- W2013678635 hasAuthorship W2013678635A5013347953 @default.
- W2013678635 hasAuthorship W2013678635A5014681063 @default.
- W2013678635 hasAuthorship W2013678635A5076966946 @default.
- W2013678635 hasConcept C112446052 @default.
- W2013678635 hasConcept C130316041 @default.
- W2013678635 hasConcept C134018914 @default.
- W2013678635 hasConcept C170493617 @default.
- W2013678635 hasConcept C181199279 @default.
- W2013678635 hasConcept C185592680 @default.
- W2013678635 hasConcept C185967709 @default.
- W2013678635 hasConcept C197902417 @default.
- W2013678635 hasConcept C2777391703 @default.
- W2013678635 hasConcept C2778336440 @default.
- W2013678635 hasConcept C2778987810 @default.
- W2013678635 hasConcept C2779306644 @default.
- W2013678635 hasConcept C41625074 @default.
- W2013678635 hasConcept C42362537 @default.
- W2013678635 hasConcept C55493867 @default.
- W2013678635 hasConcept C86803240 @default.
- W2013678635 hasConceptScore W2013678635C112446052 @default.
- W2013678635 hasConceptScore W2013678635C130316041 @default.
- W2013678635 hasConceptScore W2013678635C134018914 @default.
- W2013678635 hasConceptScore W2013678635C170493617 @default.
- W2013678635 hasConceptScore W2013678635C181199279 @default.
- W2013678635 hasConceptScore W2013678635C185592680 @default.
- W2013678635 hasConceptScore W2013678635C185967709 @default.
- W2013678635 hasConceptScore W2013678635C197902417 @default.
- W2013678635 hasConceptScore W2013678635C2777391703 @default.
- W2013678635 hasConceptScore W2013678635C2778336440 @default.
- W2013678635 hasConceptScore W2013678635C2778987810 @default.
- W2013678635 hasConceptScore W2013678635C2779306644 @default.
- W2013678635 hasConceptScore W2013678635C41625074 @default.
- W2013678635 hasConceptScore W2013678635C42362537 @default.
- W2013678635 hasConceptScore W2013678635C55493867 @default.
- W2013678635 hasConceptScore W2013678635C86803240 @default.
- W2013678635 hasIssue "1" @default.
- W2013678635 hasLocation W20136786351 @default.
- W2013678635 hasLocation W20136786352 @default.
- W2013678635 hasOpenAccess W2013678635 @default.
- W2013678635 hasPrimaryLocation W20136786351 @default.
- W2013678635 hasRelatedWork W1491267816 @default.
- W2013678635 hasRelatedWork W1862633417 @default.
- W2013678635 hasRelatedWork W2000628654 @default.
- W2013678635 hasRelatedWork W2008596330 @default.
- W2013678635 hasRelatedWork W2009923060 @default.
- W2013678635 hasRelatedWork W2033204276 @default.
- W2013678635 hasRelatedWork W2033488376 @default.
- W2013678635 hasRelatedWork W2056135496 @default.
- W2013678635 hasRelatedWork W4229758189 @default.