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• W2013681405 abstract "Human peripheral blood mononuclear lymphocytes from individuals with moderate periodontitis were separated into purified subpopulations of T lymphocytes and B lymphocytes by rosetting with sheep red blood cells (E). All three lymphocyte subpopulations were compared for proliferative responses to cell walls from seven oral bacteria, phytohemagglutinin (PHA), pokeweed mitogen (PWM), lipopolysaccharide (LPS), and streptolysin-O (SLO). Mononuclear cells and a re-combined subpopulation consisting of four parts purified T lymphocytes and one part B lymphocytes responded significantly to all of the stimulants. Purified T lymphocytes by themselves responded significantly to PHA and PWM, but were unresponsive to oral bacteria and SLO; however, T lymphocytes cultured with 2% autologous macrophages responded significantly to all seven oral bacterial cell walls and to SLO, which indicates that T-cell responses to oral bacteria are macrophage-dependent. T-cell-depleted non-E-rosette-forming B cells by themselves were poorly responsive to all of the tested stimulants; however, the responses of these cells to oral bacteria, PWM, LPS, and SLO increased significantly in the presence of 10% mitomycin-C-treated T cells, demonstrating that B cell proliferation to these stimulants is T-cell-dependent." @default.
• W2013681405 created "2016-06-24" @default.
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• W2013681405 date "1985-06-01" @default.
• W2013681405 modified "2023-10-16" @default.
• W2013681405 title "The Stimulation of Human Peripheral Blood Lymphocytes by Oral Bacteria: Macrophage and T-Cell Dependence" @default.
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• W2013681405 doi "https://doi.org/10.1177/00220345850640061001" @default.
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