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• W2013695654 abstract "The core histone mRNA levels in terminally differentiated L6 myotubes decrease to less than 5% of the amount present in proliferating myoblasts, in parallel with the cessation of DNA synthesis (Bird, R.C., Jacobs, F.A., Stein, G., Stein, J. and Sells, B.H. (1985) Biochim. Biophys. Acta 824, 209–217). The role of gene transcription in the down-shift of histone mRNA levels was assessed using a cell-free system. The level of transcription from the differentiation-independent adenovirus major late promoter was directly related to the RNA polymerase II activity of myoblast and myotube nuclear extracts. In addition, both extracts actively transcribed the histone H4 gene template containing only the 5′ proximal promoter region (−210 bp). In contrast, inclusion of the distal-proximal promoter region (−410 to −210 bp) in the template resulted in a 60% decrease in transcription by the myotube extract. A similar down-shift in transcription of the histone H3 gene template (containing 900 bp 5′ of the initiation site) by myotube nuclear extracts was also observed. The decrease in histone mRNA levels in myotubes may therefore be controlled in part by a transcriptional mechanism involving a negative regulatory factor." @default.
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• W2013695654 date "1989-11-01" @default.
• W2013695654 modified "2023-10-18" @default.
• W2013695654 title "Down-regulation of histone H3 and H4 gene transcription in differentiated L6 myotubes" @default.
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• W2013695654 doi "https://doi.org/10.1016/0167-4781(89)90098-5" @default.
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