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• W2013697439 abstract "Summary. Different commercial enzyme-linked immunsorbent assays (ELISA) were evaluated in a preliminary study for diagnosis of systemic candi- dosis: Biomericaand Virotech GmbH, which allowed immunoglobulin G detection, and Platelia � , which associated total antibody to antigen detection. They were tested with a home-made ELISA and compared with the routine techniques used in the hospital laboratory: indirect immunofluorescence and counter-immunoelectrophoresis. Sera were obtained from patients with probable or proven systemic candidosis (groups 3 and 4, n ¼ 8 and n ¼ 14, respectively) and from patients without systemic candidosis who were divided into controls (n ¼ 10), those hospitalized without Candida isolation (group 1, n ¼ 10) and those hospitalized with Candida isolation in a peripheral site (group 2, n ¼ 18). The immunoglobulin G ELISAs showed a higher sensi- tivity associated with lower specificity compared to the indirect immunofluorescence, counter-immuno- electrophoresis and total immunoglobulin ELISAs. Mannan antigen detection showed the highest specificity (78.9%). Its association with the detection of total anti-Candida immunoglobulins was more sensitive than the association of indirect immuno- fluorescence with counter-immunoelectrophoresis (95.4% versus 59%, respectively) with a specificity of 52.6% (versus 55.2%). Interest in the use of commercial ELISAs, more particularly the Platelia � tests, has to be confirmed in a prospective study with follow-up of the patients. Zusammenfassung. In einer praliminaren Stu- die uber kommerzielle ELISA-Methoden zur Serodiagnose der systemischen Candidose wurden folgende Diagnostika gepruft: Biomerica � , Virotech zum IgG-Nachweis und Plateliazum Antigen- Nachweis. Die Diagnostika wurden in einem eigenentwickelten ELISA eingesetzt und mit bishe- rigen Routinemethoden verglichen, dem indirekten Immunofluoreszenztest (IFT) und der Gegenstrom- elektrophorese (CIE). Es wurden Seren von Pati- enten mit bewiesener (n ¼ 8) oder vermuteter systemischer Candidose (n ¼ 14) gepruft sowie von Kontrollpatienten ohne Systemcandidose (n ¼ 10), Krankenhauspatienten ohne (n ¼ 10) und mit peri- pherem Candida-Nachweis (n ¼ 18). Die IgG-ELISA waren hoher sensitiv, aber weniger spezifisch als IFT, CIE und Gesamt-Ig-ELISA. Der Mannan- Antigen-Nachweis zeigte hohere Spezifitat (78.9%). Mannan-AG-Nachweis + Anti-Candida-Ig-Nachweis waren sensitiver als IFT + CIE (95.4% vs. 59%) mit einer Spezifitat von 52.6% vs. 55.2%. Der Einsatz kommerzieller ELISA-Systeme muss in prospek- tiven Follow-up-Studien noch weiter bewertet werden." @default.
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• W2013697439 date "2002-12-01" @default.
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• W2013697439 title "Evaluation of different commercial ELISA methods for the serodiagnosis of systemic candidosis - Bewertung kommerzieller ELISA-Methoden zur Serodiagnose der systemischen Candidose" @default.
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• W2013697439 doi "https://doi.org/10.1046/j.1439-0507.2002.d01-120.x" @default.
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