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- W2013720728 abstract "Abstract —Group IIA secretory nonpancreatic phospholipase A 2 (snpPLA 2 ) is associated with collagen fibers in the extracellular matrix of human atherosclerotic plaques. Decorin, a small proteoglycan (PG) carrying chondroitin/dermatan sulfate glycosaminoglycans (GAGs), forms part of the collagen network in human arteries. To explore whether snpPLA 2 may be associated with collagen fibers via interaction with decorin, we performed (1) immunohistochemistry to compare the relative in vivo localization of snpPLA 2 and decorin in human atherosclerotic tissue and (2) in vitro experiments to study the interaction between snpPLA 2 and decorin. In atherosclerotic lesions, decorin was detected within the snpPLA 2 -positive part of the intima close to the media. Electrophoretic mobility shift assay showed that snpPLA 2 binds to decorin synthesized by human fibroblasts. Native and GAG-depleted decorin enhanced the association of snpPLA 2 to collagen types I and VI in a solid-phase binding assay. Furthermore, snpPLA 2 bound efficiently to a recombinant decorin core protein fragment B/E (Asp45-Lys359). This binding was competed with soluble decorin and inhibited at NaCl concentrations >150 mmol/L. The decorin core protein fragment B/E competed better than dermatan sulfate for binding of snpPLA 2 to decorin-coated microtiter wells. The enzymatic activity of snpPLA 2 increased 2- to 3-fold in the presence of decorin or GAG-depleted decorin. The results show that snpPLA 2 binds preferentially to the decorin protein core rather than to the GAG chain and that this interaction enhances snpPLA 2 activity. As a consequence, this active extracellular enzyme may contribute to the pathogenesis of atherosclerosis by modifying lipoproteins and releasing inflammatory lipid mediators at places of lipoprotein retention in the arterial wall." @default.
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- W2013720728 date "2000-03-31" @default.
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- W2013720728 title "Molecular Basis for the Association of Group IIA Phospholipase A <sub>2</sub> and Decorin in Human Atherosclerotic Lesions" @default.
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- W2013720728 doi "https://doi.org/10.1161/01.res.86.6.707" @default.
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