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- W2013726972 abstract "Synthesis of stress proteins after heat shock and different periods of UV-B radiation were investigated with marine diatom species from the North Sea Ditylum brightwellii, Lithodesmium variabile, Odontella sinensis, Thalassiosira rotula and the Antarctic diatom Odontella weissfloggii from the Weddell Sea. Algae were grown in an artifical sea-water medium under controlled laboratory conditions: light/dark regime of 12:12 h (7.2 W m−2), normal air (0.035 vol.% CO2) and 18° or 4 °C. All the tested diatom species can produce heat shock proteins (HSPS) of the 70 kDa family by in vivo labelling with [35S]-methionine. The same results were obtained for Odontella sinensis, Ditylum brightwellii and Odontella weissflogii by estimation of the in vitro translation products with poly-A-mRNA isolated from these organisms. However, Odontella weissflogii, a species relatively insensitive to UV-B irradiance, did not synthesize UV-induced HSPS, whereas the UV-sensitive diatom Odontella sinensis, as well as Lithodesmium variabile, produced all the observed HSPS after UV-B exposure. In addition, a protein of 43 kDa was found after UV-B irradiance of the temperate Odontella sinensis. The temperate marine diatom Thalassiosira rotula synthesized 70 kDa and 5 7 kDa proteins after a heat shock and a UV-B exposure of 2 h, but a 40 kDa protein could not be detected, whereas a 60 kDa protein was found after 2 h UV-B exposure. The results are discussed in view of a possible adaptation of O. weissflogii to an enhanced UV dose." @default.
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- W2013726972 date "1995-04-01" @default.
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- W2013726972 title "Pattern of Proteins after Heat Shock and UV‐B Radiation of some Temperate Marine Diatoms and the Antarctic <i>Odontella weissflogii</i>" @default.
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- W2013726972 doi "https://doi.org/10.1111/j.1438-8677.1995.tb00837.x" @default.
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