FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013740510> ?p ?o ?g. }

• W2013740510 endingPage "411" @default.
• W2013740510 startingPage "401" @default.
• W2013740510 abstract "Stromal cell-secreted chemokines including CCL2 have been implicated in the primary tumor microenvironment, as mediators of tumor cell migration, proliferation, and angiogenesis. Expression of CCL2 and its principal receptor CCR2 was analyzed by RQ-PCR in primary tumor cells and breast cancer cell lines. Breast cancer cell lines (MDA-MB-231, T47D) were co-cultured directly on a monolayer of primary breast tumor and normal stromal cells, retrieved using EpCAM+ magnetic beads, and changes in expression of CCL2, CCR2, MMP11, ELK1, VIL2, and Ki67 detected by RQ-PCR. Epithelial cell migration and proliferation in response to stromal cell-secreted factors was also analyzed. In vivo, tumor xenografts were formed by co-injecting T47D cells with primary tumor stromal cells. Following establishment, tumors were harvested and digested, epithelial cells retrieved and analyzed by RQ-PCR. Whole tumor tissue was also analyzed by immunohistochemistry for CD31 and the VIL2 encoded protein Ezrin. Tumor stromal cells expressed significantly higher levels of CCL2 than normal cells, with no CCR2 expression detected. Primary epithelial cells and breast cancer cell lines expressed elevated CCL2, with relative expression of CCR2 found to be higher than the ligand. Interaction of breast cancer epithelial cells with primary tumor, but not normal stromal cells, stimulated increased expression of CCL2 (8-fold), ELK1 (6-fold), VIL2 (6-fold), and MMP11 (17-fold). Factors secreted by stromal cells, including CCL2, stimulated a significant increase in epithelial cell migration, with no effect on cell proliferation in vitro observed. In vivo, the presence of stromal cells resulted in tumors of increased volume, mediated at least in part through neoangiogenesis demonstrated by immunohistochemistry (CD31). Admixed tumor xenografts exhibited increased expression of Ki67, MMP11, VIL2, and ELK1. Elevated Ezrin protein was also detected, with increased cytoplasmic localization. The results presented highlight mechanisms through which breast cancer epithelial cells can harness stromal cell biology to support tumor progression." @default.
• W2013740510 created "2016-06-24" @default.
• W2013740510 creator A5000083069 @default.
• W2013740510 creator A5002084153 @default.
• W2013740510 creator A5026851409 @default.
• W2013740510 creator A5055691326 @default.
• W2013740510 creator A5068119873 @default.
• W2013740510 creator A5072420963 @default.
• W2013740510 creator A5077939705 @default.
• W2013740510 date "2011-02-23" @default.
• W2013740510 modified "2023-10-03" @default.
• W2013740510 title "Influence of stromal–epithelial interactions on breast cancer in vitro and in vivo" @default.
• W2013740510 cites W1518308640 @default.
• W2013740510 cites W1603541349 @default.
• W2013740510 cites W1963598532 @default.
• W2013740510 cites W1966501740 @default.
• W2013740510 cites W1968428572 @default.
• W2013740510 cites W1973220859 @default.
• W2013740510 cites W1974511120 @default.
• W2013740510 cites W1986986419 @default.
• W2013740510 cites W1993544519 @default.
• W2013740510 cites W1995714285 @default.
• W2013740510 cites W1999389436 @default.
• W2013740510 cites W2002145465 @default.
• W2013740510 cites W2003049491 @default.
• W2013740510 cites W2007366900 @default.
• W2013740510 cites W2012124753 @default.
• W2013740510 cites W2021582547 @default.
• W2013740510 cites W2037099316 @default.
• W2013740510 cites W2039100374 @default.
• W2013740510 cites W2039675196 @default.
• W2013740510 cites W2049820772 @default.
• W2013740510 cites W2058219230 @default.
• W2013740510 cites W2059888416 @default.
• W2013740510 cites W2062809449 @default.
• W2013740510 cites W2062932571 @default.
• W2013740510 cites W2067403399 @default.
• W2013740510 cites W2068955820 @default.
• W2013740510 cites W2078502914 @default.
• W2013740510 cites W2084445367 @default.
• W2013740510 cites W2086772583 @default.
• W2013740510 cites W2091546407 @default.
• W2013740510 cites W2101037339 @default.
• W2013740510 cites W2107277218 @default.
• W2013740510 cites W2118566883 @default.
• W2013740510 cites W2127481599 @default.
• W2013740510 cites W2151453636 @default.
• W2013740510 cites W2154453972 @default.
• W2013740510 cites W2156323704 @default.
• W2013740510 cites W2161744213 @default.
• W2013740510 cites W54801374 @default.
• W2013740510 doi "https://doi.org/10.1007/s10549-011-1410-9" @default.
• W2013740510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21344235" @default.
• W2013740510 hasPublicationYear "2011" @default.
• W2013740510 type Work @default.
• W2013740510 sameAs 2013740510 @default.
• W2013740510 citedByCount "21" @default.
• W2013740510 countsByYear W20137405102012 @default.
• W2013740510 countsByYear W20137405102013 @default.
• W2013740510 countsByYear W20137405102014 @default.
• W2013740510 countsByYear W20137405102015 @default.
• W2013740510 countsByYear W20137405102017 @default.
• W2013740510 countsByYear W20137405102018 @default.
• W2013740510 countsByYear W20137405102020 @default.
• W2013740510 countsByYear W20137405102021 @default.
• W2013740510 countsByYear W20137405102022 @default.
• W2013740510 countsByYear W20137405102023 @default.
• W2013740510 crossrefType "journal-article" @default.
• W2013740510 hasAuthorship W2013740510A5000083069 @default.
• W2013740510 hasAuthorship W2013740510A5002084153 @default.
• W2013740510 hasAuthorship W2013740510A5026851409 @default.
• W2013740510 hasAuthorship W2013740510A5055691326 @default.
• W2013740510 hasAuthorship W2013740510A5068119873 @default.
• W2013740510 hasAuthorship W2013740510A5072420963 @default.
• W2013740510 hasAuthorship W2013740510A5077939705 @default.
• W2013740510 hasBestOaLocation W20137405102 @default.
• W2013740510 hasConcept C121608353 @default.
• W2013740510 hasConcept C16930146 @default.
• W2013740510 hasConcept C2776107976 @default.
• W2013740510 hasConcept C2779013556 @default.
• W2013740510 hasConcept C2780283643 @default.
• W2013740510 hasConcept C2780394083 @default.
• W2013740510 hasConcept C3020616263 @default.
• W2013740510 hasConcept C502942594 @default.
• W2013740510 hasConcept C54355233 @default.
• W2013740510 hasConcept C86803240 @default.
• W2013740510 hasConceptScore W2013740510C121608353 @default.
• W2013740510 hasConceptScore W2013740510C16930146 @default.
• W2013740510 hasConceptScore W2013740510C2776107976 @default.
• W2013740510 hasConceptScore W2013740510C2779013556 @default.
• W2013740510 hasConceptScore W2013740510C2780283643 @default.
• W2013740510 hasConceptScore W2013740510C2780394083 @default.
• W2013740510 hasConceptScore W2013740510C3020616263 @default.
• W2013740510 hasConceptScore W2013740510C502942594 @default.
• W2013740510 hasConceptScore W2013740510C54355233 @default.
• W2013740510 hasConceptScore W2013740510C86803240 @default.
• W2013740510 hasIssue "2" @default.
• W2013740510 hasLocation W20137405101 @default.

• next