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- W2013749545 abstract "As Barenfanger and Drake illustrated in their recent article,1 direct microscopy of Gram-stained smears of clinical specimens is an extremely important part of diagnostic microbiology. In addition to what can be considered the basics, an awareness of other possibilities may be of diagnostic value. This present article illustrates some of these possibilities. Some of the findings are self-evident if the observer knows the morphological features or structures, eg, Giardia lamblia and Trichomonas vaginalis. Others are less obvious, eg, toxoplasma trophozoites, “negatively-stained” organisms, and possibly Pneumocystis carinii. For these, suspicions must be confirmed with other stains and/or techniques. Whatever the source of the specimen, heat fixation of smears is not recommended. Fixation with methanol or the ethanol/acetone mixture used as the decolorizing reagent gives less distortion and better results with the Gram stain.2,3" @default.
- W2013749545 created "2016-06-24" @default.
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- W2013749545 date "2002-08-01" @default.
- W2013749545 modified "2023-09-23" @default.
- W2013749545 title "Gram Stains – Beyond the Basics" @default.
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- W2013749545 doi "https://doi.org/10.1309/u8uv-v9ja-jygh-eyal" @default.
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