Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013759152> ?p ?o ?g. }
- W2013759152 endingPage "286" @default.
- W2013759152 startingPage "279" @default.
- W2013759152 abstract "Electrospray ionization mass spectrometry (ESI-MS) is employed to directly analyze the limited trypsinolysis products of wild-type tumor necrosis factor-α (wtTNF-α) and its mutant, M3S. To determine the charge numbers of peaks of relatively small peptides in the ESI mass spectrum of a digest, a series of sodium-adduct ion peaks of each peptide are generated by adding a small quantity of NaCl to the digest before taking the spectrum. From the monitoring of the composition of proteolytic mixture as the incubation time is lengthened, it has been learned that the proteolysis of wtTNF-α by trypsin occurs sequentially: Arg2, Arg6, Arg32, Arg31, and Arg44, and that M3S is strongly resistant to the proteolysis. Since the cleavage sequence of wtTNF-α and the mutation-induced resistance of M3S are consistent with the structural features of the proteins, we can suggest a mutant more resistant to proteolysis than M3S, which has an additional point mutation, Ala35Leu or Ala35Ile." @default.
- W2013759152 created "2016-06-24" @default.
- W2013759152 creator A5005780710 @default.
- W2013759152 creator A5012655906 @default.
- W2013759152 creator A5026519045 @default.
- W2013759152 creator A5032470636 @default.
- W2013759152 creator A5045720936 @default.
- W2013759152 creator A5058833251 @default.
- W2013759152 creator A5076532840 @default.
- W2013759152 creator A5077530208 @default.
- W2013759152 date "1999-02-01" @default.
- W2013759152 modified "2023-10-12" @default.
- W2013759152 title "Determination of the Limited Trypsinolysis Pathways of Tumor Necrosis Factor-α and Its Mutant by Electrospray Ionization Mass Spectrometry" @default.
- W2013759152 cites W1499120045 @default.
- W2013759152 cites W1964944514 @default.
- W2013759152 cites W1973127065 @default.
- W2013759152 cites W1976174962 @default.
- W2013759152 cites W1993494847 @default.
- W2013759152 cites W2008556494 @default.
- W2013759152 cites W2024508517 @default.
- W2013759152 cites W2029348572 @default.
- W2013759152 cites W2030043624 @default.
- W2013759152 cites W2034593795 @default.
- W2013759152 cites W2041358315 @default.
- W2013759152 cites W2056287079 @default.
- W2013759152 cites W2064019484 @default.
- W2013759152 cites W2066696898 @default.
- W2013759152 cites W2069735642 @default.
- W2013759152 cites W2078987292 @default.
- W2013759152 cites W2084912000 @default.
- W2013759152 cites W2086616742 @default.
- W2013759152 cites W2087668188 @default.
- W2013759152 cites W2134469483 @default.
- W2013759152 cites W2161933361 @default.
- W2013759152 cites W2167245810 @default.
- W2013759152 cites W2176151213 @default.
- W2013759152 cites W3120153401 @default.
- W2013759152 cites W97952941 @default.
- W2013759152 doi "https://doi.org/10.1006/abio.1998.2999" @default.
- W2013759152 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10036131" @default.
- W2013759152 hasPublicationYear "1999" @default.
- W2013759152 type Work @default.
- W2013759152 sameAs 2013759152 @default.
- W2013759152 citedByCount "1" @default.
- W2013759152 crossrefType "journal-article" @default.
- W2013759152 hasAuthorship W2013759152A5005780710 @default.
- W2013759152 hasAuthorship W2013759152A5012655906 @default.
- W2013759152 hasAuthorship W2013759152A5026519045 @default.
- W2013759152 hasAuthorship W2013759152A5032470636 @default.
- W2013759152 hasAuthorship W2013759152A5045720936 @default.
- W2013759152 hasAuthorship W2013759152A5058833251 @default.
- W2013759152 hasAuthorship W2013759152A5076532840 @default.
- W2013759152 hasAuthorship W2013759152A5077530208 @default.
- W2013759152 hasConcept C104317684 @default.
- W2013759152 hasConcept C108204754 @default.
- W2013759152 hasConcept C12554922 @default.
- W2013759152 hasConcept C143065580 @default.
- W2013759152 hasConcept C151730666 @default.
- W2013759152 hasConcept C162356407 @default.
- W2013759152 hasConcept C175156509 @default.
- W2013759152 hasConcept C178790620 @default.
- W2013759152 hasConcept C181199279 @default.
- W2013759152 hasConcept C185592680 @default.
- W2013759152 hasConcept C194451485 @default.
- W2013759152 hasConcept C2780340462 @default.
- W2013759152 hasConcept C2781307694 @default.
- W2013759152 hasConcept C40684141 @default.
- W2013759152 hasConcept C43369102 @default.
- W2013759152 hasConcept C43617362 @default.
- W2013759152 hasConcept C55493867 @default.
- W2013759152 hasConcept C86803240 @default.
- W2013759152 hasConceptScore W2013759152C104317684 @default.
- W2013759152 hasConceptScore W2013759152C108204754 @default.
- W2013759152 hasConceptScore W2013759152C12554922 @default.
- W2013759152 hasConceptScore W2013759152C143065580 @default.
- W2013759152 hasConceptScore W2013759152C151730666 @default.
- W2013759152 hasConceptScore W2013759152C162356407 @default.
- W2013759152 hasConceptScore W2013759152C175156509 @default.
- W2013759152 hasConceptScore W2013759152C178790620 @default.
- W2013759152 hasConceptScore W2013759152C181199279 @default.
- W2013759152 hasConceptScore W2013759152C185592680 @default.
- W2013759152 hasConceptScore W2013759152C194451485 @default.
- W2013759152 hasConceptScore W2013759152C2780340462 @default.
- W2013759152 hasConceptScore W2013759152C2781307694 @default.
- W2013759152 hasConceptScore W2013759152C40684141 @default.
- W2013759152 hasConceptScore W2013759152C43369102 @default.
- W2013759152 hasConceptScore W2013759152C43617362 @default.
- W2013759152 hasConceptScore W2013759152C55493867 @default.
- W2013759152 hasConceptScore W2013759152C86803240 @default.
- W2013759152 hasIssue "2" @default.
- W2013759152 hasLocation W20137591521 @default.
- W2013759152 hasLocation W20137591522 @default.
- W2013759152 hasOpenAccess W2013759152 @default.
- W2013759152 hasPrimaryLocation W20137591521 @default.
- W2013759152 hasRelatedWork W1984711129 @default.
- W2013759152 hasRelatedWork W2005953458 @default.
- W2013759152 hasRelatedWork W2013759152 @default.
- W2013759152 hasRelatedWork W2014605936 @default.