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• W2013765982 abstract "Intercellular coupling between cumulus cells and oocytes has been measured in vitro using intracellular markers derived from 3H-labelled choline, uridine and inositol. These compounds can only enter oocytes through junctions with cumulus cells. Choline, selected for most detailed study, is metabolized to phosphocholine and lecithin by cumulus cells and is probably transported into oocytes after phosphorylation. The effect of gonadotropins on follicles, either in vivo or in culture is to reduce, but not totally eliminate, passage of labelled markers from cumulus cells into oocytes. The hormone primarily responsible for this change in coupling, which takes 12–15 h to become evident, is FSH. Inhibition of follicular steroid synthesis using aminoglutethimide has a small but significant effect on this hormone-dependent system of intercellular communication. The advantages are emphasized of using cumulus-oocyte complexes, together with intracellular markers, to study basic properties of intercellular coupling. Present results on cumulus cell-oocyte coupling are also discussed in relation to the regulation of meiosis." @default.
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• W2013765982 date "1980-03-01" @default.
• W2013765982 modified "2023-10-15" @default.
• W2013765982 title "Measurement of intercellular coupling between oocytes and cumulus cells using intracellular markers" @default.
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• W2013765982 doi "https://doi.org/10.1016/0014-4827(80)90466-8" @default.
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