FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013783509> ?p ?o ?g. }

• W2013783509 endingPage "78" @default.
• W2013783509 startingPage "78" @default.
• W2013783509 abstract "Pathogen identification in clinical routine is based on the cultivation of microbes with subsequent morphological and physiological characterisation lasting at least 24 hours. However, early and accurate identification is a crucial requisite for fast and optimally targeted antimicrobial treatment. Molecular biology based techniques allow fast identification, however discrimination of very closely related species remains still difficult.A molecular approach is presented for the rapid identification of pathogens combining PCR amplification with microarray detection. The DNA chip comprises oligonucleotide capture probes for 25 different pathogens including Gram positive cocci, the most frequently encountered genera of Enterobacteriaceae, non-fermenter and clinical relevant Candida species. The observed detection limits varied from 10 cells (e.g. E. coli) to 10(5) cells (S. aureus) per mL artificially spiked blood. Thus the current low sensitivity for some species still represents a barrier for clinical application. Successful discrimination of closely related species was achieved by a signal pattern recognition approach based on the k-nearest-neighbour method. A prototype software providing this statistical evaluation was developed, allowing correct identification in 100 % of the cases at the genus and in 96.7 % at the species level (n = 241).The newly developed molecular assay can be carried out within 6 hours in a research laboratory from pathogen isolation to species identification. From our results we conclude that DNA microarrays can be a useful tool for rapid identification of closely related pathogens particularly when the protocols are adapted to the special clinical scenarios." @default.
• W2013783509 created "2016-06-24" @default.
• W2013783509 creator A5009240917 @default.
• W2013783509 creator A5017947992 @default.
• W2013783509 creator A5022687922 @default.
• W2013783509 creator A5044753485 @default.
• W2013783509 creator A5055337379 @default.
• W2013783509 creator A5068840357 @default.
• W2013783509 creator A5081542680 @default.
• W2013783509 creator A5085574223 @default.
• W2013783509 date "2007-01-01" @default.
• W2013783509 modified "2023-10-15" @default.
• W2013783509 title "Identification of human pathogens isolated from blood using microarray hybridisation and signal pattern recognition" @default.
• W2013783509 cites W1490697372 @default.
• W2013783509 cites W1555782211 @default.
• W2013783509 cites W1652636050 @default.
• W2013783509 cites W1696298601 @default.
• W2013783509 cites W1779346397 @default.
• W2013783509 cites W1791412297 @default.
• W2013783509 cites W1944042070 @default.
• W2013783509 cites W1975713828 @default.
• W2013783509 cites W1978613215 @default.
• W2013783509 cites W1988491479 @default.
• W2013783509 cites W1988595948 @default.
• W2013783509 cites W1991533874 @default.
• W2013783509 cites W1995656159 @default.
• W2013783509 cites W2007549716 @default.
• W2013783509 cites W2026212124 @default.
• W2013783509 cites W2027207787 @default.
• W2013783509 cites W2036322154 @default.
• W2013783509 cites W2039039849 @default.
• W2013783509 cites W2041902804 @default.
• W2013783509 cites W2045496013 @default.
• W2013783509 cites W2050168569 @default.
• W2013783509 cites W2050781380 @default.
• W2013783509 cites W2054938569 @default.
• W2013783509 cites W2055643289 @default.
• W2013783509 cites W2060199965 @default.
• W2013783509 cites W2062882143 @default.
• W2013783509 cites W2065498551 @default.
• W2013783509 cites W2077860424 @default.
• W2013783509 cites W2094747692 @default.
• W2013783509 cites W2095989309 @default.
• W2013783509 cites W2106046401 @default.
• W2013783509 cites W2107974397 @default.
• W2013783509 cites W2114022837 @default.
• W2013783509 cites W2120865735 @default.
• W2013783509 cites W2122440798 @default.
• W2013783509 cites W2123159066 @default.
• W2013783509 cites W2124181672 @default.
• W2013783509 cites W2133371096 @default.
• W2013783509 cites W2134103927 @default.
• W2013783509 cites W2134697946 @default.
• W2013783509 cites W2141963879 @default.
• W2013783509 cites W2141973151 @default.
• W2013783509 cites W2142149155 @default.
• W2013783509 cites W2144080673 @default.
• W2013783509 cites W2145224096 @default.
• W2013783509 cites W2147955113 @default.
• W2013783509 cites W2152918576 @default.
• W2013783509 cites W2154074683 @default.
• W2013783509 cites W2155806125 @default.
• W2013783509 cites W2156818800 @default.
• W2013783509 cites W2158441493 @default.
• W2013783509 cites W2159830959 @default.
• W2013783509 cites W2160510046 @default.
• W2013783509 cites W2160939145 @default.
• W2013783509 cites W2162191065 @default.
• W2013783509 cites W2164799961 @default.
• W2013783509 cites W2165823492 @default.
• W2013783509 cites W2167835550 @default.
• W2013783509 cites W2170575095 @default.
• W2013783509 cites W2183255420 @default.
• W2013783509 cites W2233527513 @default.
• W2013783509 doi "https://doi.org/10.1186/1471-2180-7-78" @default.
• W2013783509 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1994958" @default.
• W2013783509 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17697354" @default.
• W2013783509 hasPublicationYear "2007" @default.
• W2013783509 type Work @default.
• W2013783509 sameAs 2013783509 @default.
• W2013783509 citedByCount "48" @default.
• W2013783509 countsByYear W20137835092012 @default.
• W2013783509 countsByYear W20137835092013 @default.
• W2013783509 countsByYear W20137835092014 @default.
• W2013783509 countsByYear W20137835092015 @default.
• W2013783509 countsByYear W20137835092016 @default.
• W2013783509 countsByYear W20137835092018 @default.
• W2013783509 countsByYear W20137835092019 @default.
• W2013783509 countsByYear W20137835092020 @default.
• W2013783509 crossrefType "journal-article" @default.
• W2013783509 hasAuthorship W2013783509A5009240917 @default.
• W2013783509 hasAuthorship W2013783509A5017947992 @default.
• W2013783509 hasAuthorship W2013783509A5022687922 @default.
• W2013783509 hasAuthorship W2013783509A5044753485 @default.
• W2013783509 hasAuthorship W2013783509A5055337379 @default.
• W2013783509 hasAuthorship W2013783509A5068840357 @default.
• W2013783509 hasAuthorship W2013783509A5081542680 @default.
• W2013783509 hasAuthorship W2013783509A5085574223 @default.

• next