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• W2013808724 abstract "Background. Organ cold storage times may be extended by modifications to organ preservation solutions. Methods. Three preservation solutions were investigated for their ability to maintain viable hepatic bioenergetics in stored pig livers: modified University of Wisconsin (mUW); mUW+adenosine (1.34 g/L), and mUW+iloprost (10−8 mol/L), a prostacyclin analogue. Using human liver retrieval and storage techniques, pig livers were stored on ice for either 2 or 16 hr, after which phosphorus-31 spectra were collected every 2 min during the period of cold ischemia and hypothermic reperfusion (HtR). During HtR, metabolite concentration changes associated with phosphomonoesters, inorganic phosphate, γ-nucleotide triphosphate (NTP), and β-NTP were measured for all solutions. Results. After a 2-hr storage, β-NTP regeneration in mUW+iloprost produced +57.7% (P<0.01) more β-NTP, at a faster initial rate of +66.3% (P<0.001), compared with mUW, and mUW+adenosine regenerated +35.6% (P<0.05) more β-NTP, compared with mUW. Storage for 16 hr did not slow the rates of regeneration, and the total NTP produced during the course of the experiment remained unchanged for the respective preservation solutions. Cessation of HtR invoked a net accumulation of nucleotide diphosphate, indicating differential kinetics of adenine nucleotide hydrolysis. Conclusion. This large animal model study suggests significant improvements to human organ preservation solutions using prostacyclin analogues and adenosine with respect to hepatic bioenergetics." @default.
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• W2013808724 date "1999-08-01" @default.
• W2013808724 modified "2023-10-17" @default.
• W2013808724 title "IMPROVED PRESERVATION SOLUTIONS FOR ORGAN STORAGE" @default.
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• W2013808724 doi "https://doi.org/10.1097/00007890-199908150-00005" @default.
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