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- W2013818564 abstract "Affinity tags are typically used to facilitate membrane protein purification. A protease needs to remain active in the presence of detergent to remove a fusion tag from a recombinant membrane protein. Our results show that the West Nile virus (WNV) protease activity was not affected while in the presence of a wide range of detergents. In a detergent solution, the WNV protease can remove the fusion tag from a recombinant protein containing KCNE3 and a WNV protease site. Therefore, the WNV protease may be useful as an alternative enzyme to remove affinity tags in protein purifications." @default.
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- W2013818564 date "2013-04-01" @default.
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- W2013818564 title "West Nile virus protease activity in detergent solutions and application for affinity tag removal" @default.
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- W2013818564 doi "https://doi.org/10.1016/j.ab.2012.12.015" @default.
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