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• W2013825319 endingPage "867" @default.
• W2013825319 startingPage "860" @default.
• W2013825319 abstract "Aim Although activated T lymphocytes express tryptophan hydroxylase 1 and produce 5-HT, the metabolic fate and cellular handling of this 5-HT is unclear. Here, we investigated key proteins in T cells linked to 5-HT metabolism and storage and compare differences in 5-HT synthesis and metabolism between T-cell subsets. Methods We cultured human Jurkat T cells and mouse splenic CD3+, CD4+ and CD8+ T cells with or without T-cell activators (phorbol ester/ionomycin, concavalin A or plate-bound anti-CD3 antibody). Subsequently, we measured mRNA and/or protein for monoamine oxidase A and B, vesicular monoamine transporter 1 and 2, N-acetyl transferase and tryptophan hydroxylase 1. In addition, we measured the release of exogenously loaded [3H]5-HT and endogenously synthesized 5-HT from CD4+ and CD8+ T-cell subsets. Results Human and mouse T cells selectively express monoamine oxidase A. Following T-cell activation, mRNA levels of MAO-A increase robustly in parallel with tryptophan hydroxylase 1. Concomitant with these changes, T cells increase the expression of the type 1 vesicular monoamine transporter. Raised intracellular [Ca2+] rapidly releases preloaded [3H]5-HT from CD4+ and CD8+ T cells indicating that these cells have the capacity for the storage and regulated secretion of 5-HT. Notably, both the expression of tryptophan hydroxylase 1 and monoamine oxidase A, and 5-HT production are significantly greater in CD8+ compared with CD4+ T cells. Conclusion These data reveal coordinated changes in 5-HT production, metabolism and storage that may optimize 5-HT secretion from the CD8+ T cell subset in response to activation stimuli." @default.
• W2013825319 created "2016-06-24" @default.
• W2013825319 creator A5017371290 @default.
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• W2013825319 date "2015-02-28" @default.
• W2013825319 modified "2023-10-17" @default.
• W2013825319 title "T lymphocytes possess the machinery for 5-HT synthesis, storage, degradation and release" @default.
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• W2013825319 doi "https://doi.org/10.1111/apha.12470" @default.
• W2013825319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25683571" @default.
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