Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013864739> ?p ?o ?g. }
- W2013864739 endingPage "215" @default.
- W2013864739 startingPage "207" @default.
- W2013864739 abstract "Calreticulin is a 46-kDa Ca2+-binding chaperone of the endoplasmic reticulum membranes. The protein binds Ca2+ with high capacity, affects intracellular Ca2+ homeostasis, and functions as a lectin-like chaperone. In this study, we describe expression and purification procedures for the isolation of recombinant rabbit calreticulin. The calreticulin was expressed in Pichia pastoris and purified to homogeneity by DEAE–Sepharose and Resource Q FPLC chromatography. The protein was not retained in the endoplasmic reticulum of Pichia pastoris but instead it was secreted into the external media. The purification procedures reported here for recombinant calreticulin yield homogeneous preparations of the protein by SDS–PAGE and mass spectroscopy analysis. Purified calreticulin was identified by its NH2-terminal amino acid sequences, by its Ca2+ binding, and by its reactivity with anti-calreticulin antibodies. The protein contained one disulfide bond between 88Cys and 120Cys. CD spectral analysis and Ca2+-binding properties of the recombinant protein indicated that it was correctly folded." @default.
- W2013864739 created "2016-06-24" @default.
- W2013864739 creator A5052943656 @default.
- W2013864739 creator A5059473395 @default.
- W2013864739 creator A5061630360 @default.
- W2013864739 creator A5062219892 @default.
- W2013864739 creator A5062545523 @default.
- W2013864739 creator A5065768536 @default.
- W2013864739 creator A5067325703 @default.
- W2013864739 creator A5071277216 @default.
- W2013864739 date "2000-11-01" @default.
- W2013864739 modified "2023-09-23" @default.
- W2013864739 title "Expression and Purification of Mammalian Calreticulin in Pichia pastoris" @default.
- W2013864739 cites W109442298 @default.
- W2013864739 cites W139881350 @default.
- W2013864739 cites W1482605569 @default.
- W2013864739 cites W1487568705 @default.
- W2013864739 cites W1503394109 @default.
- W2013864739 cites W1570786435 @default.
- W2013864739 cites W1586011497 @default.
- W2013864739 cites W1600052472 @default.
- W2013864739 cites W1670068025 @default.
- W2013864739 cites W1775749144 @default.
- W2013864739 cites W182430618 @default.
- W2013864739 cites W1932052686 @default.
- W2013864739 cites W1968124895 @default.
- W2013864739 cites W1971349684 @default.
- W2013864739 cites W1996691196 @default.
- W2013864739 cites W1997280917 @default.
- W2013864739 cites W1997843453 @default.
- W2013864739 cites W1999466952 @default.
- W2013864739 cites W2011239271 @default.
- W2013864739 cites W2013293359 @default.
- W2013864739 cites W2017915061 @default.
- W2013864739 cites W2020006960 @default.
- W2013864739 cites W2031825312 @default.
- W2013864739 cites W2032524317 @default.
- W2013864739 cites W2037709017 @default.
- W2013864739 cites W2038341665 @default.
- W2013864739 cites W2054861954 @default.
- W2013864739 cites W2059132145 @default.
- W2013864739 cites W2060120692 @default.
- W2013864739 cites W2061964177 @default.
- W2013864739 cites W2063554961 @default.
- W2013864739 cites W2085741168 @default.
- W2013864739 cites W2090202060 @default.
- W2013864739 cites W2097511933 @default.
- W2013864739 cites W2100837269 @default.
- W2013864739 cites W2101108802 @default.
- W2013864739 cites W2107810891 @default.
- W2013864739 cites W2120459320 @default.
- W2013864739 cites W2128720297 @default.
- W2013864739 cites W2133933487 @default.
- W2013864739 cites W2153609006 @default.
- W2013864739 cites W2155151775 @default.
- W2013864739 cites W2179803393 @default.
- W2013864739 cites W3024054742 @default.
- W2013864739 doi "https://doi.org/10.1006/prep.2000.1291" @default.
- W2013864739 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11049745" @default.
- W2013864739 hasPublicationYear "2000" @default.
- W2013864739 type Work @default.
- W2013864739 sameAs 2013864739 @default.
- W2013864739 citedByCount "16" @default.
- W2013864739 countsByYear W20138647392012 @default.
- W2013864739 countsByYear W20138647392014 @default.
- W2013864739 countsByYear W20138647392015 @default.
- W2013864739 countsByYear W20138647392016 @default.
- W2013864739 countsByYear W20138647392017 @default.
- W2013864739 countsByYear W20138647392019 @default.
- W2013864739 countsByYear W20138647392020 @default.
- W2013864739 countsByYear W20138647392023 @default.
- W2013864739 crossrefType "journal-article" @default.
- W2013864739 hasAuthorship W2013864739A5052943656 @default.
- W2013864739 hasAuthorship W2013864739A5059473395 @default.
- W2013864739 hasAuthorship W2013864739A5061630360 @default.
- W2013864739 hasAuthorship W2013864739A5062219892 @default.
- W2013864739 hasAuthorship W2013864739A5062545523 @default.
- W2013864739 hasAuthorship W2013864739A5065768536 @default.
- W2013864739 hasAuthorship W2013864739A5067325703 @default.
- W2013864739 hasAuthorship W2013864739A5071277216 @default.
- W2013864739 hasConcept C104317684 @default.
- W2013864739 hasConcept C122229402 @default.
- W2013864739 hasConcept C142724271 @default.
- W2013864739 hasConcept C153911025 @default.
- W2013864739 hasConcept C158617107 @default.
- W2013864739 hasConcept C178790620 @default.
- W2013864739 hasConcept C179247698 @default.
- W2013864739 hasConcept C181199279 @default.
- W2013864739 hasConcept C185592680 @default.
- W2013864739 hasConcept C2775962898 @default.
- W2013864739 hasConcept C2781372317 @default.
- W2013864739 hasConcept C40767141 @default.
- W2013864739 hasConcept C47450691 @default.
- W2013864739 hasConcept C519063684 @default.
- W2013864739 hasConcept C553089730 @default.
- W2013864739 hasConcept C55493867 @default.
- W2013864739 hasConcept C71924100 @default.
- W2013864739 hasConcept C86803240 @default.