Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013898997> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2013898997 endingPage "269" @default.
- W2013898997 startingPage "265" @default.
- W2013898997 abstract "A modified method for the measurement of proteolytic enzyme activity in cell culture-conditioned media has been developed. Using the release of 3H-labeled peptides from 3H-labeled gelatin the method is performed in microwell plates. The substrate is insolubilized and attached to the wells by glutaraldehyde treatment, thus eliminating the need for a precipitation step at the end of the assay. The assay is sensitive, reproducible, and convenient for small sample volumes. The effect of different protease inhibitors on activity can be assessed rapidly allowing an early characterization of the enzyme. It can also be adapted to microplate spectrophotometric analysis by staining residual substrate with Coomassie blue." @default.
- W2013898997 created "2016-06-24" @default.
- W2013898997 creator A5036575075 @default.
- W2013898997 creator A5061522022 @default.
- W2013898997 date "1990-03-01" @default.
- W2013898997 modified "2023-09-25" @default.
- W2013898997 title "A radiolabel-release microwell assay for proteolytic enzymes present in cell culture media" @default.
- W2013898997 cites W2010267991 @default.
- W2013898997 cites W2017648919 @default.
- W2013898997 cites W2034766013 @default.
- W2013898997 cites W2064676416 @default.
- W2013898997 cites W2068850000 @default.
- W2013898997 cites W2079831464 @default.
- W2013898997 cites W2082868338 @default.
- W2013898997 cites W2088129685 @default.
- W2013898997 cites W2341487655 @default.
- W2013898997 doi "https://doi.org/10.1016/0003-2697(90)90290-p" @default.
- W2013898997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2111103" @default.
- W2013898997 hasPublicationYear "1990" @default.
- W2013898997 type Work @default.
- W2013898997 sameAs 2013898997 @default.
- W2013898997 citedByCount "9" @default.
- W2013898997 countsByYear W20138989972013 @default.
- W2013898997 crossrefType "journal-article" @default.
- W2013898997 hasAuthorship W2013898997A5036575075 @default.
- W2013898997 hasAuthorship W2013898997A5061522022 @default.
- W2013898997 hasConcept C160845552 @default.
- W2013898997 hasConcept C181199279 @default.
- W2013898997 hasConcept C185592680 @default.
- W2013898997 hasConcept C18903297 @default.
- W2013898997 hasConcept C2776714187 @default.
- W2013898997 hasConcept C2777289219 @default.
- W2013898997 hasConcept C2777529624 @default.
- W2013898997 hasConcept C2778823387 @default.
- W2013898997 hasConcept C2780761556 @default.
- W2013898997 hasConcept C3019223276 @default.
- W2013898997 hasConcept C43617362 @default.
- W2013898997 hasConcept C54355233 @default.
- W2013898997 hasConcept C55493867 @default.
- W2013898997 hasConcept C74864618 @default.
- W2013898997 hasConcept C86803240 @default.
- W2013898997 hasConceptScore W2013898997C160845552 @default.
- W2013898997 hasConceptScore W2013898997C181199279 @default.
- W2013898997 hasConceptScore W2013898997C185592680 @default.
- W2013898997 hasConceptScore W2013898997C18903297 @default.
- W2013898997 hasConceptScore W2013898997C2776714187 @default.
- W2013898997 hasConceptScore W2013898997C2777289219 @default.
- W2013898997 hasConceptScore W2013898997C2777529624 @default.
- W2013898997 hasConceptScore W2013898997C2778823387 @default.
- W2013898997 hasConceptScore W2013898997C2780761556 @default.
- W2013898997 hasConceptScore W2013898997C3019223276 @default.
- W2013898997 hasConceptScore W2013898997C43617362 @default.
- W2013898997 hasConceptScore W2013898997C54355233 @default.
- W2013898997 hasConceptScore W2013898997C55493867 @default.
- W2013898997 hasConceptScore W2013898997C74864618 @default.
- W2013898997 hasConceptScore W2013898997C86803240 @default.
- W2013898997 hasIssue "2" @default.
- W2013898997 hasLocation W20138989971 @default.
- W2013898997 hasLocation W20138989972 @default.
- W2013898997 hasOpenAccess W2013898997 @default.
- W2013898997 hasPrimaryLocation W20138989971 @default.
- W2013898997 hasRelatedWork W2013898997 @default.
- W2013898997 hasRelatedWork W2044181871 @default.
- W2013898997 hasRelatedWork W2073250359 @default.
- W2013898997 hasRelatedWork W2118347714 @default.
- W2013898997 hasRelatedWork W2143168635 @default.
- W2013898997 hasRelatedWork W2158369850 @default.
- W2013898997 hasRelatedWork W2359423029 @default.
- W2013898997 hasRelatedWork W2361567234 @default.
- W2013898997 hasRelatedWork W2383677694 @default.
- W2013898997 hasRelatedWork W3010056926 @default.
- W2013898997 hasVolume "185" @default.
- W2013898997 isParatext "false" @default.
- W2013898997 isRetracted "false" @default.
- W2013898997 magId "2013898997" @default.
- W2013898997 workType "article" @default.