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• W2013902010 abstract "Fluorescence internal contents mixing assay was used to monitor the fusion of phosphatidylserine (PS) small unilamellar vesicles, initiated by metal ions (Ca 2+ , La 3+ and Tb 3+ ), at various concentrations of monovalent cations (Li + , Na + and K + ). The influence of ionic strength (0.02–1.0 M) on the threshold concentratino of “fusogenic” cations required to induce fusion was measured. The threshold concentrations increased monotonically (1 mM at 0.1 M to 3.1 mM at 1 M) with the increasing ionic strength of the solution for Ca 2+ , but remained unchanged for both La 3+ and Tb 3+ . Changes in the ionic strength of the encapsulated solution did not alter the threshold concentrations for all the ions studied, in the range 0.02–0.3 M. The results are analyzed in terms of competitive binding between the monovalent ions and the “fusogenic” ions (Ca 2+ , Tb 3+ and La 3+ ). It is shown that there is a critical value for calcium bound-PS, below which no massive fusion occurs. Bound and free fractions of PS are calculated based on the Gouy-Chapman model, taking activities rather than concentrations of metal ions into account. Our experiments also show that monovalent ions alone do not induce fusion even at high concentrations." @default.
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• W2013902010 date "1988-11-01" @default.
• W2013902010 modified "2023-10-16" @default.
• W2013902010 title "Effect of monovalent cations on polyvalent cation-induced fusion of phosphatidylserine small unilamellar vesicles" @default.
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• W2013902010 doi "https://doi.org/10.1016/0009-3084(88)90059-x" @default.
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