FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013927372> ?p ?o ?g. }

• W2013927372 endingPage "988" @default.
• W2013927372 startingPage "976" @default.
• W2013927372 abstract "The genomes of all retroviruses contain sequences near their 5' ends that interact with the nucleocapsid domains (NC) of assembling Gag proteins and direct their packaging into virus particles. Retroviral packaging signals often occur in non-contiguous segments spanning several hundred nucleotides of the RNA genome, confounding structural and mechanistic studies of genome packaging. Recently, a relatively short, 82 nucleotide region of the Rous sarcoma virus (RSV) genome, called muPsi, was shown to be sufficient to direct efficient packaging of heterologous RNAs into RSV-like particles. We have developed a method for the preparation and purification of large quantities of recombinant RSV NC protein, and have studied its interactions with native and mutant forms of the muPsi encapsidation element. NC does not bind with significant affinity to truncated forms of muPsi, consistent with earlier packaging and mutagenesis studies. Surprisingly, NC binds to the native muPsi RNA with affinity that is approximately 100 times greater than that observed for other previously characterized retroviral NC-RNA complexes (extrapolated dissociation constant K(d)=1.9 nM). Tight binding with 1:1 NC-muPsi stoichiometry is dependent on a conserved UGCG tetraloop in one of three predicted stem loops, and an AUG initiation codon controvertibly implicated in genome packaging and translational control. Loop nucleotides of other stem loops do not contribute to NC binding. Our findings indicate that the structural determinants of RSV genome recognition and NC-RNA binding differ considerably from those observed for other retroviruses." @default.
• W2013927372 created "2016-06-24" @default.
• W2013927372 creator A5002027818 @default.
• W2013927372 creator A5003376619 @default.
• W2013927372 creator A5042107338 @default.
• W2013927372 creator A5053547522 @default.
• W2013927372 date "2005-06-01" @default.
• W2013927372 modified "2023-09-28" @default.
• W2013927372 title "High Affinity Nucleocapsid Protein Binding to the μΨ RNA Packaging Signal of Rous Sarcoma Virus" @default.
• W2013927372 cites W1442231 @default.
• W2013927372 cites W1482139092 @default.
• W2013927372 cites W1485871310 @default.
• W2013927372 cites W1492183414 @default.
• W2013927372 cites W1498439983 @default.
• W2013927372 cites W1516946230 @default.
• W2013927372 cites W153121970 @default.
• W2013927372 cites W1546979502 @default.
• W2013927372 cites W1547223088 @default.
• W2013927372 cites W1589497217 @default.
• W2013927372 cites W1599509724 @default.
• W2013927372 cites W1600831089 @default.
• W2013927372 cites W1624691696 @default.
• W2013927372 cites W1692102943 @default.
• W2013927372 cites W1727214066 @default.
• W2013927372 cites W1806273468 @default.
• W2013927372 cites W1840514170 @default.
• W2013927372 cites W1870661610 @default.
• W2013927372 cites W1893630959 @default.
• W2013927372 cites W1901653080 @default.
• W2013927372 cites W1973901059 @default.
• W2013927372 cites W1985124981 @default.
• W2013927372 cites W1985138898 @default.
• W2013927372 cites W1992726195 @default.
• W2013927372 cites W1993541478 @default.
• W2013927372 cites W2006640104 @default.
• W2013927372 cites W2009818318 @default.
• W2013927372 cites W2016719543 @default.
• W2013927372 cites W2020357919 @default.
• W2013927372 cites W2022798600 @default.
• W2013927372 cites W2027622202 @default.
• W2013927372 cites W2028653352 @default.
• W2013927372 cites W2033648662 @default.
• W2013927372 cites W2034578852 @default.
• W2013927372 cites W2038470175 @default.
• W2013927372 cites W2041121767 @default.
• W2013927372 cites W2046769835 @default.
• W2013927372 cites W2053847144 @default.
• W2013927372 cites W2055202327 @default.
• W2013927372 cites W2059663095 @default.
• W2013927372 cites W2067481787 @default.
• W2013927372 cites W2071103483 @default.
• W2013927372 cites W2075984281 @default.
• W2013927372 cites W2082176639 @default.
• W2013927372 cites W2085272777 @default.
• W2013927372 cites W2095385679 @default.
• W2013927372 cites W2096985241 @default.
• W2013927372 cites W2097653439 @default.
• W2013927372 cites W2101779661 @default.
• W2013927372 cites W2105140840 @default.
• W2013927372 cites W2116360269 @default.
• W2013927372 cites W2116450753 @default.
• W2013927372 cites W2122273986 @default.
• W2013927372 cites W2126301468 @default.
• W2013927372 cites W2126971607 @default.
• W2013927372 cites W2139143247 @default.
• W2013927372 cites W2141157874 @default.
• W2013927372 cites W2158977548 @default.
• W2013927372 cites W2165256239 @default.
• W2013927372 cites W2169821755 @default.
• W2013927372 cites W2171474432 @default.
• W2013927372 cites W4211178428 @default.
• W2013927372 cites W4233882118 @default.
• W2013927372 doi "https://doi.org/10.1016/j.jmb.2005.04.046" @default.
• W2013927372 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15907938" @default.
• W2013927372 hasPublicationYear "2005" @default.
• W2013927372 type Work @default.
• W2013927372 sameAs 2013927372 @default.
• W2013927372 citedByCount "30" @default.
• W2013927372 countsByYear W20139273722013 @default.
• W2013927372 countsByYear W20139273722014 @default.
• W2013927372 countsByYear W20139273722015 @default.
• W2013927372 countsByYear W20139273722016 @default.
• W2013927372 countsByYear W20139273722019 @default.
• W2013927372 countsByYear W20139273722020 @default.
• W2013927372 countsByYear W20139273722021 @default.
• W2013927372 countsByYear W20139273722023 @default.
• W2013927372 crossrefType "journal-article" @default.
• W2013927372 hasAuthorship W2013927372A5002027818 @default.
• W2013927372 hasAuthorship W2013927372A5003376619 @default.
• W2013927372 hasAuthorship W2013927372A5042107338 @default.
• W2013927372 hasAuthorship W2013927372A5053547522 @default.
• W2013927372 hasConcept C104317684 @default.
• W2013927372 hasConcept C141231307 @default.
• W2013927372 hasConcept C153911025 @default.
• W2013927372 hasConcept C153957851 @default.
• W2013927372 hasConcept C159047783 @default.
• W2013927372 hasConcept C2522874641 @default.
• W2013927372 hasConcept C2776294106 @default.

• next