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- W2013954192 abstract "The precision, accuracy, detection limit and peanut matrix influence of an ELISA were analysed in the determination of aflatoxin B 1 . The assay was performed on two different reference samples: peanut extract and peanut paste, that were spiked with known amounts of aflatoxins. The lower detectable level was 0.5 w g kg -1 . The average intra-assay precision expressed as coefficient of variation (CV) was 11.7% for concentrations between 2.54 and 901 w g kg -1 and the average inter-assay precision was 29.7% for the same range of concentrations. The average accuracy measured by a recovery assay in samples that contained only aflatoxin B 1 was 107%. The correlation between the ELISA and high-performance liquid chromatography (HPLC) applied to 28 peanut samples artificially contaminated with Aspergillus flavus and A. parasiticus spores showed a high correlation ( r = 0.977, P < 0.0001). The detection limits and matrix influence associated with our ELISA procedure were more sensitive than those reported for other ELISA procedures due to specific antiserum treatment." @default.
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- W2013954192 date "2002-09-01" @default.
- W2013954192 modified "2023-09-26" @default.
- W2013954192 title "Determination of Aflatoxin B 1 in Highly Contaminated Peanut Samples Using HPLC and ELISA" @default.
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- W2013954192 doi "https://doi.org/10.1080/09540100220145000" @default.
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