FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013963199> ?p ?o ?g. }

• W2013963199 endingPage "2943" @default.
• W2013963199 startingPage "2937" @default.
• W2013963199 abstract "Intramuscular creatine plays a crucial role in maintaining skeletal muscle energy homeostasis, and its entry into the cell is dependent upon the sodium chloride dependent Creatine Transporter (CrT; Slc6a8). CrT activity is regulated by a number of factors including extra- and intracellular creatine concentrations, hormones, changes in sodium concentration, and kinase activity, however very little is known about the regulation of CrT gene expression. The present study aimed to investigate how Creatine Transporter (CrT) gene expression is regulated in skeletal muscle. Within the first intron of the CrT gene, we identified a conserved sequence that includes the motif recognized by the Estrogen-related receptor α (ERRα), also known as an Estrogen-related receptor response element (ERRE). Additional ERREs confirming to the known consensus sequence were also identified in the region upstream of the promoter. When partnered with peroxisome proliferator-activated receptor-gamma co-activator-1alpha (PGC-1α) or beta (PGC-1β), ERRα induces the expression of many genes important for cellular bioenergetics. We therefore hypothesized that PGC-1 and ERRα could also regulate CrT gene expression and creatine uptake in skeletal muscle. Here we show that adenoviral overexpression of PGC-1α or PGC-1β in L6 myotubes increased CrT mRNA (2.1 and 1.7-fold, P < 0.0125) and creatine uptake (1.8 and 1.6-fold, P < 0.0125), and this effect was inhibited with co-expression of shRNA for ERRα. Overexpression of a constitutively active ERRα (VP16-ERRα) increased CrT mRNA approximately 8-fold (P < 0.05), resulting in a 2.2-fold (P < 0.05) increase in creatine uptake. Lastly, chromatin immunoprecipitation assays revealed that PGC-1α and ERRα directly interact with the CrT gene and increase CrT gene expression." @default.
• W2013963199 created "2016-06-24" @default.
• W2013963199 creator A5022613720 @default.
• W2013963199 creator A5023258982 @default.
• W2013963199 creator A5032616673 @default.
• W2013963199 creator A5033333025 @default.
• W2013963199 creator A5042629542 @default.
• W2013963199 creator A5044318232 @default.
• W2013963199 creator A5091675350 @default.
• W2013963199 date "2014-12-01" @default.
• W2013963199 modified "2023-10-15" @default.
• W2013963199 title "PGC-1α and PGC-1β increase CrT expression and creatine uptake in myotubes via ERRα" @default.
• W2013963199 cites W105155487 @default.
• W2013963199 cites W1565150924 @default.
• W2013963199 cites W1598394864 @default.
• W2013963199 cites W1605945811 @default.
• W2013963199 cites W1969384498 @default.
• W2013963199 cites W1974086574 @default.
• W2013963199 cites W1978454641 @default.
• W2013963199 cites W1990579890 @default.
• W2013963199 cites W1993337559 @default.
• W2013963199 cites W1994878901 @default.
• W2013963199 cites W1996058126 @default.
• W2013963199 cites W1996821192 @default.
• W2013963199 cites W2021610728 @default.
• W2013963199 cites W2026443722 @default.
• W2013963199 cites W2028946095 @default.
• W2013963199 cites W2034175329 @default.
• W2013963199 cites W2035456940 @default.
• W2013963199 cites W2046099131 @default.
• W2013963199 cites W2049481764 @default.
• W2013963199 cites W2052989289 @default.
• W2013963199 cites W2053266431 @default.
• W2013963199 cites W2063516253 @default.
• W2013963199 cites W2066159720 @default.
• W2013963199 cites W2075610661 @default.
• W2013963199 cites W2077645437 @default.
• W2013963199 cites W2080536099 @default.
• W2013963199 cites W2082984525 @default.
• W2013963199 cites W2083408882 @default.
• W2013963199 cites W2083447148 @default.
• W2013963199 cites W2083456009 @default.
• W2013963199 cites W2086668012 @default.
• W2013963199 cites W2087729479 @default.
• W2013963199 cites W2089256281 @default.
• W2013963199 cites W2092512352 @default.
• W2013963199 cites W2095005830 @default.
• W2013963199 cites W2096857133 @default.
• W2013963199 cites W2104801001 @default.
• W2013963199 cites W2116242674 @default.
• W2013963199 cites W2118644362 @default.
• W2013963199 cites W2119966340 @default.
• W2013963199 cites W2120293566 @default.
• W2013963199 cites W2123106337 @default.
• W2013963199 cites W2131492087 @default.
• W2013963199 cites W2132056205 @default.
• W2013963199 cites W2134567067 @default.
• W2013963199 cites W2135164358 @default.
• W2013963199 cites W2135645000 @default.
• W2013963199 cites W2137507155 @default.
• W2013963199 cites W2156976744 @default.
• W2013963199 cites W2157199218 @default.
• W2013963199 cites W2161424531 @default.
• W2013963199 cites W2162234541 @default.
• W2013963199 cites W2165547205 @default.
• W2013963199 cites W2166706354 @default.
• W2013963199 cites W2170903888 @default.
• W2013963199 cites W2177449395 @default.
• W2013963199 cites W4233093956 @default.
• W2013963199 cites W4252584570 @default.
• W2013963199 doi "https://doi.org/10.1016/j.bbamcr.2014.08.010" @default.
• W2013963199 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25173818" @default.
• W2013963199 hasPublicationYear "2014" @default.
• W2013963199 type Work @default.
• W2013963199 sameAs 2013963199 @default.
• W2013963199 citedByCount "18" @default.
• W2013963199 countsByYear W20139631992015 @default.
• W2013963199 countsByYear W20139631992016 @default.
• W2013963199 countsByYear W20139631992017 @default.
• W2013963199 countsByYear W20139631992018 @default.
• W2013963199 countsByYear W20139631992019 @default.
• W2013963199 countsByYear W20139631992020 @default.
• W2013963199 countsByYear W20139631992021 @default.
• W2013963199 countsByYear W20139631992023 @default.
• W2013963199 crossrefType "journal-article" @default.
• W2013963199 hasAuthorship W2013963199A5022613720 @default.
• W2013963199 hasAuthorship W2013963199A5023258982 @default.
• W2013963199 hasAuthorship W2013963199A5032616673 @default.
• W2013963199 hasAuthorship W2013963199A5033333025 @default.
• W2013963199 hasAuthorship W2013963199A5042629542 @default.
• W2013963199 hasAuthorship W2013963199A5044318232 @default.
• W2013963199 hasAuthorship W2013963199A5091675350 @default.
• W2013963199 hasBestOaLocation W20139631991 @default.
• W2013963199 hasConcept C104317684 @default.
• W2013963199 hasConcept C126322002 @default.
• W2013963199 hasConcept C134018914 @default.
• W2013963199 hasConcept C150194340 @default.
• W2013963199 hasConcept C173396325 @default.

• next