FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013977277> ?p ?o ?g. }

• W2013977277 endingPage "236" @default.
• W2013977277 startingPage "227" @default.
• W2013977277 abstract "Lewis phenotyping by hemagglutination is an unreliable routine method for Lewis antigen designation. Now genomic typing of the Lewis gene is available. Additionally, flow cytometry has been used for typing. We wanted to compare the results of Lewis typing in healthy individuals using the three methods.Ninety-three randomly selected plasma donors were genotyped for inactivating Secretor (FUT2) G428A and Lewis (FUT3) T59G, T202C, C314T, G508A and T1067A point mutations. All Le(a+b-) individuals (nonsecretors) were homozygous for the FUT2 G428A mutation and all Le(a-b-) individuals had inactivating mutations on both FUT3 alleles. Fixed erythrocytes were analyzed by fluorescence-activated flow cytometry and the results were compared with hem- agglutination and genotypic data. Antigen availability was expressed as median fluorescence intensity and as percentage positive cells with fluorescence intensities > or =10(2).Using an anti-Le(a) reagent a mean of 99% of erythrocytes from Le(a+b-) individuals and 1% of erythrocytes from Le(a-b-) or Le(a-b+) individuals were stained positive. Using an anti-Le(b) reagent, a mean of 71% of erythrocytes from A(1), 95% from B and 99% from O and A(2) Le(a-b+) individuals and less than 10% of erythrocytes from Le(a-b-) or Le(a+b-) individuals were stained positive. After papain treatment 100% of the erythrocytes from A(1) and A(1)B Le(a-b+) individuals stained positive without increase in background staining. The flow cytometric technique revealed large differences in staining intensities, within each ABO Le(a-b+) subgroup which was not directly correlated to plasma donation frequencies nor to Secretor or Lewis genotypes.Flow cytometry may prove valuable as a Lewis blood group typing technique but also as a research tool when investigating Lewis phenotypes of human erythrocytes." @default.
• W2013977277 created "2016-06-24" @default.
• W2013977277 creator A5022316371 @default.
• W2013977277 creator A5033075070 @default.
• W2013977277 creator A5055116623 @default.
• W2013977277 creator A5077160595 @default.
• W2013977277 creator A5082864510 @default.
• W2013977277 date "1999-01-01" @default.
• W2013977277 modified "2023-09-23" @default.
• W2013977277 title "Typing for the Human Lewis Blood Group System by Quantitative Fluorescence–Activated Flow Cytometry: Large Differences in Antigen Presentation on Erythrocytes between A₁, A₂, B, O Phenotypes" @default.
• W2013977277 cites W1022571150 @default.
• W2013977277 cites W1480461444 @default.
• W2013977277 cites W1539344862 @default.
• W2013977277 cites W1558984579 @default.
• W2013977277 cites W1595705455 @default.
• W2013977277 cites W1933205066 @default.
• W2013977277 cites W1968226729 @default.
• W2013977277 cites W1978968433 @default.
• W2013977277 cites W1982964418 @default.
• W2013977277 cites W1997805546 @default.
• W2013977277 cites W1997993215 @default.
• W2013977277 cites W2010011251 @default.
• W2013977277 cites W2016590404 @default.
• W2013977277 cites W2018046970 @default.
• W2013977277 cites W2018628288 @default.
• W2013977277 cites W2030336958 @default.
• W2013977277 cites W2034270672 @default.
• W2013977277 cites W2041693836 @default.
• W2013977277 cites W2047394974 @default.
• W2013977277 cites W2047440121 @default.
• W2013977277 cites W2058322693 @default.
• W2013977277 cites W2061211069 @default.
• W2013977277 cites W2071531790 @default.
• W2013977277 cites W2074443036 @default.
• W2013977277 cites W2074629823 @default.
• W2013977277 cites W2078179498 @default.
• W2013977277 cites W2079657455 @default.
• W2013977277 cites W2086947927 @default.
• W2013977277 cites W2089803113 @default.
• W2013977277 cites W2094438579 @default.
• W2013977277 cites W2105311240 @default.
• W2013977277 cites W2136911663 @default.
• W2013977277 cites W2138368229 @default.
• W2013977277 cites W2145343046 @default.
• W2013977277 cites W2151734103 @default.
• W2013977277 cites W2401833872 @default.
• W2013977277 cites W2700239370 @default.
• W2013977277 cites W1976496028 @default.
• W2013977277 doi "https://doi.org/10.1159/000031132" @default.
• W2013977277 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10717603" @default.
• W2013977277 hasPublicationYear "1999" @default.
• W2013977277 type Work @default.
• W2013977277 sameAs 2013977277 @default.
• W2013977277 citedByCount "16" @default.
• W2013977277 countsByYear W20139772772017 @default.
• W2013977277 countsByYear W20139772772020 @default.
• W2013977277 crossrefType "journal-article" @default.
• W2013977277 hasAuthorship W2013977277A5022316371 @default.
• W2013977277 hasAuthorship W2013977277A5033075070 @default.
• W2013977277 hasAuthorship W2013977277A5055116623 @default.
• W2013977277 hasAuthorship W2013977277A5077160595 @default.
• W2013977277 hasAuthorship W2013977277A5082864510 @default.
• W2013977277 hasConcept C143508840 @default.
• W2013977277 hasConcept C147483822 @default.
• W2013977277 hasConcept C153911025 @default.
• W2013977277 hasConcept C176080213 @default.
• W2013977277 hasConcept C185592680 @default.
• W2013977277 hasConcept C203014093 @default.
• W2013977277 hasConcept C2781209916 @default.
• W2013977277 hasConcept C54355233 @default.
• W2013977277 hasConcept C553184892 @default.
• W2013977277 hasConcept C74864618 @default.
• W2013977277 hasConcept C86803240 @default.
• W2013977277 hasConceptScore W2013977277C143508840 @default.
• W2013977277 hasConceptScore W2013977277C147483822 @default.
• W2013977277 hasConceptScore W2013977277C153911025 @default.
• W2013977277 hasConceptScore W2013977277C176080213 @default.
• W2013977277 hasConceptScore W2013977277C185592680 @default.
• W2013977277 hasConceptScore W2013977277C203014093 @default.
• W2013977277 hasConceptScore W2013977277C2781209916 @default.
• W2013977277 hasConceptScore W2013977277C54355233 @default.
• W2013977277 hasConceptScore W2013977277C553184892 @default.
• W2013977277 hasConceptScore W2013977277C74864618 @default.
• W2013977277 hasConceptScore W2013977277C86803240 @default.
• W2013977277 hasIssue "4" @default.
• W2013977277 hasLocation W20139772771 @default.
• W2013977277 hasLocation W20139772772 @default.
• W2013977277 hasOpenAccess W2013977277 @default.
• W2013977277 hasPrimaryLocation W20139772771 @default.
• W2013977277 hasRelatedWork W1976685831 @default.
• W2013977277 hasRelatedWork W1994629536 @default.
• W2013977277 hasRelatedWork W2032754370 @default.
• W2013977277 hasRelatedWork W2055520121 @default.
• W2013977277 hasRelatedWork W2382436848 @default.
• W2013977277 hasRelatedWork W2416531657 @default.
• W2013977277 hasRelatedWork W3013381930 @default.
• W2013977277 hasRelatedWork W3032393435 @default.
• W2013977277 hasRelatedWork W4285403907 @default.

• next