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- W2013982406 abstract "We have devised a novel method to visualize the fluorescence spectrum of a single fluorescent molecule using prism‐based spectroscopy. Equiping a total internal reflection microscope with a newly designed wedge prism, we obtained a spectral image of a single rhodamine red molecule attached to an essential light chain of myosin. We also obtained a spectral image of single‐pair fluorescence resonance energy transfer between rhodamine red and Cy5 in a double‐labeled myosin motor domain. This method could become a useful tool to investigate the dynamic processes of biomolecules at the single‐molecule level." @default.
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- W2013982406 date "2002-01-23" @default.
- W2013982406 modified "2023-10-09" @default.
- W2013982406 title "Imaging of the fluorescence spectrum of a single fluorescent molecule by prism-based spectroscopy" @default.
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- W2013982406 doi "https://doi.org/10.1016/s0014-5793(02)02269-x" @default.
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