FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013983411> ?p ?o ?g. }

• W2013983411 endingPage "700" @default.
• W2013983411 startingPage "691" @default.
• W2013983411 abstract "The DLP12 lysis cassette (essD, ybcT, rzpD/rzoD) is required in certain Escherichia coli strains for normal curli expression and biofilm development. Tightly controlled regulation of the lysis cassette is of particular importance, since its overexpression causes host cell lysis. In silico analysis revealed a putative intrinsic transcriptional terminator 100 bp upstream of essD and within 2000 bp of ybcQ (Q(DLP12)), a putative lambda (λ) Q-like antiterminator. We hypothesized that Q(DLP12) may be required for effective expression of the lysis cassette. In this work we report on the role of Q(DLP12) as a positive regulator of DLP12 lysis cassette expression. Mutants lacking Q(DLP12) exhibited a biofilm-defective phenotype analogous to that of the lysis cassette knockouts. This defect occurred through the downregulation of curli transcription, which is also consistent with that seen in the lysis cassette mutants and was restored by complementation by ectopic expression of Q(DLP12). In addition, Q(DLP12) overexpression caused cell lysis, as demonstrated by leakage of β-galactosidase activity from cells. This was accompanied by upregulation of the DLP12 lysis cassette as demonstrated by increased essD transcription, which was documented with gfp-reporter assays, RT-PCR and chromatin immunoprecipitation (ChIP). We provide evidence that this Q-mediated effect resulted from direct interaction of Q(DLP12) with the lysis cassette promoter (essDp), as demonstrated by electrophoretic gel mobility shift assay (EMSA). We propose that Q(DLP12) encodes a functional transcriptional regulator, which promotes expression of the DLP12 lysis cassette. This work provides evidence of a regulator from a defective prophage affecting host cell physiology." @default.
• W2013983411 created "2016-06-24" @default.
• W2013983411 creator A5000536603 @default.
• W2013983411 creator A5024172558 @default.
• W2013983411 creator A5025314023 @default.
• W2013983411 creator A5038142961 @default.
• W2013983411 creator A5064963353 @default.
• W2013983411 date "2013-04-01" @default.
• W2013983411 modified "2023-09-24" @default.
• W2013983411 title "A Q-like transcription factor regulates biofilm development in Escherichia coli by controlling expression of the DLP12 lysis cassette" @default.
• W2013983411 cites W1511395722 @default.
• W2013983411 cites W1540505601 @default.
• W2013983411 cites W1579416801 @default.
• W2013983411 cites W1598639912 @default.
• W2013983411 cites W1767304651 @default.
• W2013983411 cites W1871973308 @default.
• W2013983411 cites W1921607560 @default.
• W2013983411 cites W1936255557 @default.
• W2013983411 cites W1967862819 @default.
• W2013983411 cites W1996064131 @default.
• W2013983411 cites W2004614746 @default.
• W2013983411 cites W2006462246 @default.
• W2013983411 cites W2019147544 @default.
• W2013983411 cites W2036917159 @default.
• W2013983411 cites W2039016483 @default.
• W2013983411 cites W2040445629 @default.
• W2013983411 cites W2045403483 @default.
• W2013983411 cites W2049230354 @default.
• W2013983411 cites W2051375960 @default.
• W2013983411 cites W2055294982 @default.
• W2013983411 cites W2059018132 @default.
• W2013983411 cites W2070068738 @default.
• W2013983411 cites W2079872549 @default.
• W2013983411 cites W2080410350 @default.
• W2013983411 cites W2085679553 @default.
• W2013983411 cites W2105173502 @default.
• W2013983411 cites W2107743271 @default.
• W2013983411 cites W2109335319 @default.
• W2013983411 cites W2116137883 @default.
• W2013983411 cites W2117357746 @default.
• W2013983411 cites W2118606775 @default.
• W2013983411 cites W2122608498 @default.
• W2013983411 cites W2122922894 @default.
• W2013983411 cites W2122992192 @default.
• W2013983411 cites W2130023752 @default.
• W2013983411 cites W2130132655 @default.
• W2013983411 cites W2136036642 @default.
• W2013983411 cites W2138502991 @default.
• W2013983411 cites W2142612355 @default.
• W2013983411 cites W2143606104 @default.
• W2013983411 cites W2148357087 @default.
• W2013983411 cites W2162335168 @default.
• W2013983411 cites W2162623417 @default.
• W2013983411 cites W2167007274 @default.
• W2013983411 cites W2167356927 @default.
• W2013983411 cites W2168167613 @default.
• W2013983411 cites W2168486098 @default.
• W2013983411 cites W2170825389 @default.
• W2013983411 cites W4211043921 @default.
• W2013983411 doi "https://doi.org/10.1099/mic.0.064741-0" @default.
• W2013983411 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23378572" @default.
• W2013983411 hasPublicationYear "2013" @default.
• W2013983411 type Work @default.
• W2013983411 sameAs 2013983411 @default.
• W2013983411 citedByCount "4" @default.
• W2013983411 countsByYear W20139834112015 @default.
• W2013983411 countsByYear W20139834112016 @default.
• W2013983411 countsByYear W20139834112021 @default.
• W2013983411 countsByYear W20139834112022 @default.
• W2013983411 crossrefType "journal-article" @default.
• W2013983411 hasAuthorship W2013983411A5000536603 @default.
• W2013983411 hasAuthorship W2013983411A5024172558 @default.
• W2013983411 hasAuthorship W2013983411A5025314023 @default.
• W2013983411 hasAuthorship W2013983411A5038142961 @default.
• W2013983411 hasAuthorship W2013983411A5064963353 @default.
• W2013983411 hasConcept C100012643 @default.
• W2013983411 hasConcept C10147152 @default.
• W2013983411 hasConcept C101762097 @default.
• W2013983411 hasConcept C104317684 @default.
• W2013983411 hasConcept C127561419 @default.
• W2013983411 hasConcept C134320426 @default.
• W2013983411 hasConcept C138885662 @default.
• W2013983411 hasConcept C143065580 @default.
• W2013983411 hasConcept C150194340 @default.
• W2013983411 hasConcept C153911025 @default.
• W2013983411 hasConcept C179926584 @default.
• W2013983411 hasConcept C183978625 @default.
• W2013983411 hasConcept C188082640 @default.
• W2013983411 hasConcept C2776650774 @default.
• W2013983411 hasConcept C40767141 @default.
• W2013983411 hasConcept C41895202 @default.
• W2013983411 hasConcept C54355233 @default.
• W2013983411 hasConcept C547475151 @default.
• W2013983411 hasConcept C57409179 @default.
• W2013983411 hasConcept C86339819 @default.
• W2013983411 hasConcept C86803240 @default.
• W2013983411 hasConcept C92087593 @default.
• W2013983411 hasConcept C95444343 @default.

• next