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- W2013986970 abstract "Fucose containing structural glycoproteins of early embryonic chick hearts were shown to be only slightly solubilized (30% recovery) by LiCl, urea, ammonium acetate, SDS or reduction with dithiothreitol and alkylation with iodoacetamide. Solubilization is essentially complete (90% recovery) if reduction and alkylation takes place in the presence of SDS or if native material is digested with testicular hyaluronidase. These data suggest that newly synthesized embryonic cardiac structural glycoproteins become incorporated into the matrix complex with other components both covalently and non-covalently. By electrophoretic criteria there are seemingly relatively few molecular species of glycoproteins composed of glycopeptides that can be divided into four major groups on the basis of charge. Because of the interactions between matrix macromolecules the properties, hence biological function, may change as a result of changes in interactions as well as composition. Fucose containing structural glycoproteins of early embryonic chick hearts were shown to be only slightly solubilized (30% recovery) by LiCl, urea, ammonium acetate, SDS or reduction with dithiothreitol and alkylation with iodoacetamide. Solubilization is essentially complete (90% recovery) if reduction and alkylation takes place in the presence of SDS or if native material is digested with testicular hyaluronidase. These data suggest that newly synthesized embryonic cardiac structural glycoproteins become incorporated into the matrix complex with other components both covalently and non-covalently. By electrophoretic criteria there are seemingly relatively few molecular species of glycoproteins composed of glycopeptides that can be divided into four major groups on the basis of charge. Because of the interactions between matrix macromolecules the properties, hence biological function, may change as a result of changes in interactions as well as composition." @default.
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- W2013986970 date "1977-06-01" @default.
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- W2013986970 title "Structural glycoproteins of the embryonic cardiac extracellular matrix" @default.
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- W2013986970 doi "https://doi.org/10.1016/s0022-2828(77)80024-2" @default.
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