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- W2014000587 abstract "ABSTRACT A model laboratory oilfield reservoir system consisting of a packed sand thermal gradient under continuous seawater infiltration was constructed to study the migration, persistence and activity of sulfate-reducing bacteria (SRB). Nutrients (fatty acids and NH4+/PO4−3) or biocide (glutaraldehyde) were added to the injection water to determine optimum conditions for SRB growth and control of microbial H2S formation. Halotolerant SRB isolated from Gulf Coast seawater were injected into the packed sand to promote colonization. Data obtained from several experiments demonstrated that: 1) there is no apparent correlation between the numbers of viable SRB recovered in a waterflood and the presence of H2S levels, 2) SRB which grow optimally at 25 and 35°C are thermo-tolerant and could be recovered from higher temperatures of the gradient (55 and 75°C). 3)Microbial sulfide production in the seawater and formation water injection fluid occurred only when lactic acid substrate was present, 4)H2S levels generated in the packed sand varied from 50–150 mg/day (30–100 mg/1 column effluent), 5) SRB growth and activity in the seawater flood could be significantly eliminated by the addition of high biocide concentrations (> 1000 mg/1 glutaraldehyde) and 6) SRB produced H2S that was depleted in δ34S from the seawater SO4−2 by 10–12 per mil (δ34S = +6 to +8). These results show that sulfidogenic bacteria injected into a reservoir with the flood water may survive higher temperatures in the formation and be recovered from producing well fluids. These organisms may colonize cooler zones and sustain growth by degrading fatty acids in formation waters. Furthermore, recent literature findings on the isolation of extremely thermophilic fatty acid-utilizing sulfate-reducers (grow at 70–90°C) and SRB capable of growing on saturated hydrocarbons (C12 – C16) from oilfield production systems and anaerobic sediments suggests that thermophilic oil degraders may also be responsible for the onset of sour gas production in initially sweet waterfloods." @default.
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- W2014000587 date "1993-03-02" @default.
- W2014000587 modified "2023-09-27" @default.
- W2014000587 title "Growth and Control of Sulfidogenic Bacteria in a Laboratory Model Seawater Flood Thermal Gradient" @default.
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- W2014000587 doi "https://doi.org/10.2118/25198-ms" @default.
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