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- W2014006617 abstract "Frozen cryosections were cut parallel to the surface of porcine skin and palatal, buccal and floor-of-mouth mucosa so as to provide separate samples representing various epithelial layers. The samples were dried, extracted with chloroform: methanol, and the lipids were chromatographed on silica gel plates in various solvent systems. After charring, lipids were quantified with a scanning densitometer. Overall, greater differences in proportions and distributions of lipid components were evident between keratinized and non-keratinized epithelia than between epidermis and keratinized oral epithelium. For epidermis and palate there was an increase in neutral lipids, including ceramides, from the deeper layers to the surface; ceramides were most abundant in surface layers. In buccal epithelium there was a distinct increase in glycosylceramides toward the surface, and in both non-keratinized regions ceramides were present in only very small amounts. The results suggest that although neutral lipids may be associated with a superficial barrier layer in skin and oral mucosa, there are differences in the composition of this barrier between keratinized and non-keratinized epithelia." @default.
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- W2014006617 date "1991-01-01" @default.
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- W2014006617 title "Thin-layer chromatographic analyses of lipids in different layers of porcine epidermis and oral epithelium" @default.
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- W2014006617 doi "https://doi.org/10.1016/0003-9969(91)90017-o" @default.
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