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- W2014010167 abstract "The Alzheimer field has for long been divided into two hypotheses, the Amyloid-β (Aβ) hypothesis, and the Tau hypothesis. It is now becoming evident that there is a role for both proteins in the development of Alzheimer′s disease and an increasing amount of evidence indicates that there is a need for abnormalities in both Aβ and Tau for development of a full scale Alzheimer disease. There seems to be a link between Aβ up-regulation and Tau phosphorylation and further investigation of this is important for a deeper understanding of the disease. A correlation between the amounts of Aβ, Tau and phosphorylated Tau (p-Tau) in cerebrospinal fluid has been proposed as a valuable diagnostic tool for Alzheimer's disease. Simultaneously detection of Aβ, Tau and p-Tau (181) can be made using commercially available Luminex xMAP technique. The Luminex technique, by which you can measure the amount of several peptides/proteins in one sample, saves time and sample size compared to other techniques such as ELISA or Western BLot. Our aim was to modify the Innogenetics Luminex kit to work not only with cerebrospinal fluid, but also with cell lysates, as a tool for cell culture research on the correlations between Aβ and Tau phosphorylation. SH-SY5Y neuroblastoma cells were harvested and lysed with the Bioplex cell lysis kit. Protein concentration was determined and the cell lysate was diluted into a working concentration. The method was performed according to the kit instructions (Innogenetics, INNO-BIA AlzBio3) with slight modifications. The results obtained from the Luminex method was compared with results obtained from an Elisa Aβ 1–42 kit (for Aβ) and Western blot using the 5A6 antibody (total tau) and a p-181 Tau antibody (for p-Tau). Our results so far provides support for the use of the Innogenetics Inno-Bia AlzBio3 Luminex kit for measurements of Aβ, Tau and p-Tau 181 in cell lysate. This represents a sample- and timesaving method for in vitro investigations of correlations of Aβ and Tau phosphorylation in Alzheimer's disease." @default.
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- W2014010167 date "2008-07-01" @default.
- W2014010167 modified "2023-09-27" @default.
- W2014010167 title "P1-130: Simultaneous quantification of intracellular a-beta, Tau and P-Tau (181) using luminex X-map technology" @default.
- W2014010167 doi "https://doi.org/10.1016/j.jalz.2008.05.717" @default.
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