FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014016687> ?p ?o ?g. }

• W2014016687 endingPage "1719" @default.
• W2014016687 startingPage "1710" @default.
• W2014016687 abstract "Although homeostatic disturbance of the blood pH and calcium in the vicinity of tissue injury/malignancy/local infection seems subtle, it can cause substantial pathophysiological consequences, a phenomenon which has remained largely unexplored. The fibrinogen-related proteins (FREPs) containing fibrinogen-like domain (FBG) represent a conserved protein family with a common calcium-binding region, implying the presence of elements responsive to physiological perturbation. Here, we studied the molecular interaction between a representative FREP, the M-ficolin, and an acute phase blood protein, the C-reactive protein (CRP), both of which are known to trigger and control seminal pathways in infection and injury. Using hydrogen-deuterium exchange mass spectrometry, we showed that the C-terminal region of M-ficolin FBG underwent dramatic conformational change upon pH and calcium perturbations. Biochemical and biophysical assays showed that under defined pathophysiological condition (pH 6.5, 2.0 mM calcium), the FBG:CRP interaction occurred more strongly compared to that under physiological condition (pH 7.4, 2.5 mM calcium). We identified the binding interface between CRP and FBG, locating it to the pH- and calcium-sensitive C-terminal region of FBG. By site-directed mutagenesis, we determined H284 in the N-acetylglucosamine (GlcNAc)-binding pocket of the FBG, to be the critical CRP-binding residue. This conformational switch involving H284, explains how the pathophysiologically-driven FBG:CRP interaction diverts the M-ficolin away from GlcNAc/pathogen-recognition to host protein-protein interaction, thus enabling the host to regain homeostatic control. Our elucidation of the binding interface at the flexible FBG domain provides insights into the bioactive centre of the M-ficolin, and possibly other FREPs, which might aid future development of immunomodulators." @default.
• W2014016687 created "2016-06-24" @default.
• W2014016687 creator A5016756488 @default.
• W2014016687 creator A5025588089 @default.
• W2014016687 creator A5033904419 @default.
• W2014016687 creator A5062156792 @default.
• W2014016687 creator A5081786054 @default.
• W2014016687 date "2011-10-01" @default.
• W2014016687 modified "2023-10-10" @default.
• W2014016687 title "Pathophysiological condition changes the conformation of a flexible FBG-related protein, switching it from pathogen-recognition to host-interaction" @default.
• W2014016687 cites W1542648807 @default.
• W2014016687 cites W17446229 @default.
• W2014016687 cites W1811909808 @default.
• W2014016687 cites W1818037853 @default.
• W2014016687 cites W1903961798 @default.
• W2014016687 cites W1923704062 @default.
• W2014016687 cites W1964340982 @default.
• W2014016687 cites W1966052933 @default.
• W2014016687 cites W1966603445 @default.
• W2014016687 cites W1971066039 @default.
• W2014016687 cites W1979320568 @default.
• W2014016687 cites W1981317794 @default.
• W2014016687 cites W1993398535 @default.
• W2014016687 cites W1996364990 @default.
• W2014016687 cites W1999300370 @default.
• W2014016687 cites W2001453076 @default.
• W2014016687 cites W2003803079 @default.
• W2014016687 cites W2008397550 @default.
• W2014016687 cites W2012105542 @default.
• W2014016687 cites W2014117331 @default.
• W2014016687 cites W2015158463 @default.
• W2014016687 cites W2021926340 @default.
• W2014016687 cites W2024541829 @default.
• W2014016687 cites W2040175795 @default.
• W2014016687 cites W2045312983 @default.
• W2014016687 cites W2047513823 @default.
• W2014016687 cites W2047522139 @default.
• W2014016687 cites W2048521619 @default.
• W2014016687 cites W2049701119 @default.
• W2014016687 cites W2050936113 @default.
• W2014016687 cites W2057053216 @default.
• W2014016687 cites W2059610330 @default.
• W2014016687 cites W2063572291 @default.
• W2014016687 cites W2064773757 @default.
• W2014016687 cites W2065725205 @default.
• W2014016687 cites W2090640877 @default.
• W2014016687 cites W2092008869 @default.
• W2014016687 cites W2100596063 @default.
• W2014016687 cites W2114320763 @default.
• W2014016687 cites W2123797660 @default.
• W2014016687 cites W2139240444 @default.
• W2014016687 cites W2147024375 @default.
• W2014016687 cites W2148812205 @default.
• W2014016687 cites W2152304760 @default.
• W2014016687 cites W2170805498 @default.
• W2014016687 cites W229017931 @default.
• W2014016687 cites W4253569579 @default.
• W2014016687 doi "https://doi.org/10.1016/j.biochi.2011.06.003" @default.
• W2014016687 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21689722" @default.
• W2014016687 hasPublicationYear "2011" @default.
• W2014016687 type Work @default.
• W2014016687 sameAs 2014016687 @default.
• W2014016687 citedByCount "12" @default.
• W2014016687 countsByYear W20140166872012 @default.
• W2014016687 countsByYear W20140166872013 @default.
• W2014016687 countsByYear W20140166872014 @default.
• W2014016687 countsByYear W20140166872015 @default.
• W2014016687 countsByYear W20140166872017 @default.
• W2014016687 countsByYear W20140166872022 @default.
• W2014016687 crossrefType "journal-article" @default.
• W2014016687 hasAuthorship W2014016687A5016756488 @default.
• W2014016687 hasAuthorship W2014016687A5025588089 @default.
• W2014016687 hasAuthorship W2014016687A5033904419 @default.
• W2014016687 hasAuthorship W2014016687A5062156792 @default.
• W2014016687 hasAuthorship W2014016687A5081786054 @default.
• W2014016687 hasConcept C10162356 @default.
• W2014016687 hasConcept C122229402 @default.
• W2014016687 hasConcept C12554922 @default.
• W2014016687 hasConcept C134018914 @default.
• W2014016687 hasConcept C178790620 @default.
• W2014016687 hasConcept C185592680 @default.
• W2014016687 hasConcept C2779036427 @default.
• W2014016687 hasConcept C519063684 @default.
• W2014016687 hasConcept C55493867 @default.
• W2014016687 hasConcept C63645605 @default.
• W2014016687 hasConcept C86803240 @default.
• W2014016687 hasConcept C87975464 @default.
• W2014016687 hasConcept C95444343 @default.
• W2014016687 hasConceptScore W2014016687C10162356 @default.
• W2014016687 hasConceptScore W2014016687C122229402 @default.
• W2014016687 hasConceptScore W2014016687C12554922 @default.
• W2014016687 hasConceptScore W2014016687C134018914 @default.
• W2014016687 hasConceptScore W2014016687C178790620 @default.
• W2014016687 hasConceptScore W2014016687C185592680 @default.
• W2014016687 hasConceptScore W2014016687C2779036427 @default.
• W2014016687 hasConceptScore W2014016687C519063684 @default.
• W2014016687 hasConceptScore W2014016687C55493867 @default.
• W2014016687 hasConceptScore W2014016687C63645605 @default.

• next