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- W2014039097 abstract "MethylCap-seq is a robust procedure for genome-wide profiling of DNA methylation. The approach consists of the capture of methylated DNA using the MBD domain of MeCP2, and subsequent next-generation sequencing of eluted DNA. Elution of the captured methylated DNA is done in steps using a salt gradient, which stratifies the genome into fractions with different CpG density. The enrichment reached within the individual eluates allows for cost-effective deep sequence coverage. The profiles together yield a detailed genome-wide map of methylated regions and readily allows detection of DNA methylation in known and novel regions. Here, we describe principles and details of the MethylCap-seq procedure using different sources of starting material." @default.
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- W2014039097 date "2010-11-01" @default.
- W2014039097 modified "2023-10-02" @default.
- W2014039097 title "Whole-genome DNA methylation profiling using MethylCap-seq" @default.
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- W2014039097 doi "https://doi.org/10.1016/j.ymeth.2010.06.012" @default.
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